Author

D. Boassa

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Cell Biology· 5 Biophysics· 2 Biochemistry· 1 Bioengineering· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 6 Organism:human· 4 Research Focus:cell type annotation· 3 Assay:electronic health records· 3 Research Focus:protein folding & dynamics· 3 Assay:proteomics· 3 Assay:fluorescence / live-cell microscopy· 2 Assay:imaging· 2

Frequent Co-Authors

M. H. Ellisman· 6 shared S. Phan· 6 shared J. Hu· 5 shared S. R. Adams· 3 shared E. A. Bushong· 2 shared G. A. Castillon· 2 shared J. T. Ngo· 2 shared R. Ramachandra· 2 shared

Activity by Year

2018-2023 · peak 4/yr

Co-Author Collaboration Network

10 top collaborators · 32 mutual co-author links

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6 shared papersCell Biology

M. H. Ellisman

Mutual co-authors in cluster

Author profile →

9 papers

Recent·Most cited

Fe-TAMLs as a new class of small molecule peroxidase probes for correlated light and electron microscopy

S. R. Adams, M. R. Mackey, R. Ramachandra, T. J. Deerinck, G. A. Castillon, S. Phan, J. Hu, D. Boassa, J. T. Ngo, M. H. Ellisman

The paper introduces Fe-TAML, a small molecule peroxidase probe for correlated light and electron microscopy, demonstrating its use in imaging DNA, tagged proteins, and endogenous proteins.

bioRxiv · Aug 25, 2023 · PreprintCell Biology

Proximal Molecular Probe Transfer (PROMPT), a new approach for identifying sites of protein/nucleic acid interaction in cells by correlated light and electron microscopy

G. A. Castillon, S. Phan, J. Hu, D. Boassa, S. R. Adams, M. H. Ellisman

The paper introduces PROMPT, a method to identify protein/nucleic acid interaction sites using correlated light and electron microscopy, demonstrating its application with histone H2B and Fibrillarin.

bioRxiv · May 30, 2023Cell Biology

Unconventional tonicity-regulated nuclear trafficking of NFAT5 mediated by KPNB1, XPOT and RUVBL2

C. Y. Cheung, T.-T. Huang, N. Chow, S. Zhang, Y. Zhao, C. C. Wong, D. Boassa, S. Phan, M. H. Ellisman, J. R. Yates, S. Xu, Z. Yu, Y. Zhang, R. Zhang, L. L. Ng, B. C. B. Ko

This study identifies KPNB1 and RUVBL2 as key regulators of NFAT5's unconventional tonicity-dependent nuclear trafficking.

bioRxiv · Jul 11, 2021Cell Biology

Conformation and dynamics of the kinase domain drive subcellular location and activation of LRRK2

S. H. Schmidt, J.-H. Weng, P. C. Aoto, D. Boassa, S. Mathea, S. Silletti, J. Hu, M. Wallbott, E. A. Komives, S. Knapp, F. W. Herberg, S. S. Taylor

This study investigates how Parkinson's Disease mutations affect LRRK2 activation and subcellular localization through conformational changes and domain interactions.

bioRxiv · Jul 13, 2020Biochemistry

The in situ structure of Parkinson’s disease-linked LRRK2

R. Watanabe, R. Buschauer, J. Boehning, M. Audagnotto, K. Lasker, T. W. Lu, D. Boassa, S. Taylor, E. Villa

This study uses cryo-electron tomography to reveal the in situ structure of LRRK2 with a pathogenic mutation, showing its oligomerization around microtubules and functional domains.

bioRxiv · Nov 10, 2019Biophysics

Directed evolution of split APEX peroxidase

Y. Han, J. Martell, T. Branon, D. Boassa, D. Shechner, M. Ellisman, A. Ting

This study describes the directed evolution of a split APEX peroxidase, demonstrating its reconstitution and functionality in different cellular contexts.

bioRxiv · Oct 31, 2018 · PreprintBioengineering

Split-miniSOG for detecting and localizing intracellular protein-protein interactions: application to correlated light and electron microscopy

D. BOASSA, S. P. Lemieux, V. Lev-Ram, J. Hu, Q. Xiong, S. Phan, M. Mackey, R. Ramachandra, S. R. Adams, R. Y. Tsien, M. H. Ellisman, J. T. Ngo

The paper introduces Split-miniSOG, a PCA method to detect and localize PPIs using correlated light and electron microscopy.

bioRxiv · Sep 21, 2018Cell Biology

CDeep3M - Plug-and-Play cloud based deep learning for image segmentation of light, electron and X-ray microscopy

M. Haberl, C. Churas, L. Tindall, D. Boassa, S. Phan, E. A. Bushong, M. Madany, R. Akay, T. J. Deerinck, S. T. Peltier, M. H. Ellisman

CDeep3M provides a cloud-based deep learning tool for image segmentation across light, electron, and X-ray microscopy, simplifying access and computation for researchers.

bioRxiv · Jun 21, 2018Cell Biology

High-quality ultrastructural preservation using cryofixation for 3D electron microscopy of genetically labeled tissues

T. K. Tsang, E. A. Bushong, D. Boassa, J. Hu, B. Romoli, S. Phan, D. Dulcis, C.-Y. Su, M. H. Ellisman

This paper develops a method to enable genetically labeled cryofixed samples to be analyzed with SBEM and 3D CLEM, overcoming previous technical limitations.

bioRxiv · Feb 7, 2018Biophysics