Dana L. Carper

Engineered Pseudomonas putida reconfigures metabolic fluxes to support energy demands during muconate bioproduction from lignin-related aromatics

This paper investigates how engineered Pseudomonas putida reconfigures metabolic fluxes to enhance muconate production from lignin-related aromatics.

Genome shuffling enables quantitative trait locus mapping in Bacillus subtilis

This study uses genome shuffling in Bacillus subtilis to map quantitative trait loci for complex phenotypes, identifying high-confidence QTLs of moderate size.

Cellular Stress Tolerance Governs Genetic Transformability in Recalcitrant Candida Species

This study identifies cellular stress tolerance as a key factor in genetic transformability of Candida species and develops a method to improve CRISPR screening efficiency.

Structural and oligomeric characterization of substrate- and product-selective nylon hydrolases

This study characterizes three nylon hydrolases, identifying structural features and activity differences that inform their substrate specificity and potential for engineering.

Improved nylon polymerization using amide diads

This paper demonstrates a method to produce higher-molecular-weight nylons using chemically-synthesized diads and biosynthetic amide synthetases, expanding the range of usable precursors.

Process optimization for enhanced enzymatic nylon deconstruction

This study optimizes reaction conditions for three nylon-degrading enzymes, achieving high product titers for PA6 and PA66 without enzyme engineering or substrate pretreatment, and demonstrates scalability for complex PA-based materials.

Regioselective biosynthesis of oligoamides as precursors for sequence-controlled co-polyamides

This study demonstrates that NIS synthetases can synthesize sequenced oligoamides from unprotected substrates, enabling the creation of novel sequence-defined polyamides with improved properties.

Proteome Reallocation Reveals Dynamics and Mechanisms of Phage Infection and Defense in Staphylococcus aureus

This study uses longitudinal proteomics to analyze how Staphylococcus aureus reallocates resources during phage infection, revealing mechanisms of host defense and phage evasion.

Identification and characterization of substrate- and product-selective nylon hydrolases

The paper identifies and characterizes diverse nylon hydrolases, showing that 40% of tested enzymes have significant activity, with some showing substrate selectivity for Nylon 6,6 over Nylon 6.

Microbial Modulation of Host Plant Proteasome Activity Improves Heat Stress Tolerance

This paper identifies ECM29 as a key protein mediating microbial enhancement of plant heat stress tolerance through proteasome modulation in Arabidopsis.

High-density CRISPRi screens reveal adaptive transcriptional gradients in cyanobacteria

This study uses CRISPRi screens to identify transcriptional gradients in Synechococcus sp. PCC 7002 that improve growth under cold monochromatic light conditions.