Author

G. J. Knott

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 4 Bioengineering· 1 Cancer Biology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 4 Research Focus:drug response / drug discovery· 2 Organism:human· 2 Approach:wet-lab / biochemical assay· 2 Organism:bacteria (other)· 1 Assay:bulk RNA-seq· 1 Research Focus:cancer & tumor microenvironment· 1 Research Focus:cell signaling & trafficking· 1

Frequent Co-Authors

J. A. Doudna· 5 shared J. C. Cofsky· 2 shared K. M. Soczek· 2 shared A. Asif· 1 shared A. East-Seletsky· 1 shared A. M. Amen· 1 shared A. M. McKinney· 1 shared A. Mancini· 1 shared

Activity by Year

2018-2022 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 18 mutual co-author links

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5 shared papersBiochemistry

J. A. Doudna

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Author profile →

6 papers

Recent·Most cited

Crystal structure of an RNA/DNA strand exchange junction

J. C. Cofsky, G. J. Knott, C. L. Gee, J. A. Doudna

The paper reports the X-ray crystal structure of an RNA/DNA strand exchange junction, revealing structural principles of A-to-B helical transitions in RNA/DNA hybrids.

bioRxiv · Jan 24, 2022Biochemistry

CRISPR-Cas9 bends and twists DNA to read its sequence

J. C. Cofsky, K. M. Soczek, G. J. Knott, E. Nogales, J. A. Doudna

This paper reveals how CRISPR-Cas9 bends and twists DNA to flip nucleotides toward guide RNA for sequence interrogation.

bioRxiv · Sep 6, 2021Biochemistry

Engineering improved Cas13 effectors for targeted post-transcriptional regulation of gene expression

E. J. Charles, S. E. Kim, G. J. Knott, D. Smock, J. Doudna, D. F. Savage

This paper engineers improved Cas13 effectors for targeted post-transcriptional regulation, showing efficient translational repression in bacteria and exon skipping in human cells.

bioRxiv · May 26, 2021 · PreprintBioengineering

Cancer-specific loss of TERT activation sensitizes glioblastoma to DNA damage

A. M. Amen, C. Fellmann, K. M. Soczek, S. M. Ren, R. J. Lew, G. J. Knott, J. E. Park, A. M. McKinney, A. Mancini, J. A. Doudna, J. F. Costello

This study shows that reducing GABPB1L in TERT-mutant glioblastomas, combined with chemotherapy, reduces tumor growth by impairing DNA damage response.

Proceedings of the National Academy of Sciences · Mar 23, 2021Peer-reviewedCancer Biology

Machine Learning Predicts New Anti-CRISPR Proteins

S. Eitzinger, A. Asif, K. E. Watters, A. T. Iavarone, G. J. Knott, J. A. Doudna, F. u. A. A. Minhas

This paper introduces AcRanker, a machine learning method that predicts new anti-CRISPR proteins from proteomes, identifying two novel candidates through sequence analysis.

bioRxiv · Nov 29, 2019Biochemistry

RNA binding and HEPN nuclease activation are decoupled in CRISPR-Cas13a

A. Tambe, A. East-Seletsky, G. J. Knott, J. A. Doudna, M. R. O'Connell

This study shows that RNA binding and nuclease activation in CRISPR-Cas13a are separate processes, with distinct requirements for guide-target base pairing.

bioRxiv · Apr 16, 2018Biochemistry