H. B. Brandao

Dynamics of CTCF and cohesin mediated chromatin looping revealed by live-cell imaging

This study uses live-cell imaging to show that chromatin loops at the Fbn2 TAD are rare and dynamic, challenging the notion of stable TADs.

DNA double-strand break end synapsis by DNA loop extrusion

This study shows that DNA loop extrusion, with targeted loading and stabilization, enables efficient DSB end synapsis, challenging the assumption of passive diffusion.

MCM complexes are barriers that restrict cohesin-mediated loop extrusion

The paper shows MCM complexes block cohesin-mediated loop extrusion, reducing CTCF-anchored loops and TAD boundary insulation in G1 phase.

Chromosome organization by one-sided and two-sided loop extrusion

This study investigates one-sided and two-sided loop extrusion by SMC complexes, showing that one-sided extrusion cannot recapitulate in vivo observations but variants can, suggesting SMC complexes may function as two-sided motors or have biased loading.

RNA polymerases as moving barriers to condensin loop extrusion

This study quantitatively investigates how RNA polymerases interact with condensin loop extrusion, showing RNAPs act as steric barriers that impede the process, particularly in highly transcribed regions.