Author

I. M. Dobbie

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biophysics· 4 Cell Biology· 3 Bioengineering· 1 Microbiology· 1 Scientific Communication & Education· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 8 Assay:fluorescence / live-cell microscopy· 6 Assay:imaging· 6 Approach:microscopy & imaging analysis· 3 Research Focus:software / tool· 3 Research Focus:cell type annotation· 2 Research Focus:benchmarking· 1 Research Focus:cell signaling & trafficking· 1

Frequent Co-Authors

I. Davis· 7 shared M. A. Phillips· 6 shared M. J. Booth· 6 shared R. M. Parton· 5 shared M. Harkiolaki· 3 shared N. J. Hall· 3 shared D. I. Stuart· 2 shared D. M. S. Pinto· 2 shared

Activity by Year

2019-2024 · peak 5/yr

Co-Author Collaboration Network

10 top collaborators · 37 mutual co-author links

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7 shared papersCell Biology

I. Davis

Mutual co-authors in cluster

Author profile →

10 papers

Recent·Most cited

Applying 3D correlative structured illumination microscopy and X-ray tomography to characterise herpes simplex virus-1 morphogenesis

K. L. Nahas, V. Connor, K. J. Wijesinghe, H. G. Barrow, I. M. Dobbie, M. Harkiolaki, S. C. Graham, C. M. Crump

This study uses 3D correlative fluorescence and X-ray tomography to analyze HSV-1 mutants, revealing roles of viral proteins in morphogenesis and showing envelopment occurs via budding.

bioRxiv · Mar 13, 2024Microbiology

Python-Microscope: A new open source Python library for the control of microscopes

D. M. M. S. Susano Pinto, M. A. Phillips, N. Hall, J. Mateos-Langerak, D. Stoychev, T. S. Pinto, M. J. Booth, I. Davis, I. M. Dobbie

Python-Microscope provides a scalable, open-source Python library for controlling complex custom microscopes and simulating microscope systems.

bioRxiv · Jan 18, 2021Biophysics

Microscope-Cockpit: Python-based bespoke microscopy for bio-medical science

M. A. Phillips, D. M. Susano Pinto, N. Hall, J. Mateos-Langerak, R. M. Parton, D. V. Stoychev, T. Park, T. S. Pinto, J. W. Sedat, M. J. Booth, I. Davis, I. M. Dobbie

The paper introduces Microscope-Cockpit, a Python-based GUI for controlling bespoke microscopes, enabling efficient specimen navigation and hardware integration through open-source tools.

bioRxiv · Jan 18, 2021Biophysics

Wavefront-sensorless adaptive optics with a laser-free spinning disk confocal microscope

S. A. Hussain, T. Kubo, N. J. Hall, D. Gala, K. Hampson, R. M. Parton, M. A. Phillips, M. Wincott, K. Fujita, I. Davis, I. M. Dobbie, M. J. Booth

The paper introduces wavefront-sensorless adaptive optics for a laser-free spinning disk confocal microscope, improving signal, contrast, and resolution by correcting specimen-induced aberrations.

bioRxiv · Jul 28, 2020Cell Biology

Microscope-AOtools: A generalised adaptive optics implementation

N. J. Hall, J. Titlow, M. J. Booth, I. M. Dobbie

Microscope-AOtools provides a generalised, easy-to-use software package for implementing adaptive optics in biological imaging to correct aberrations and improve image quality.

bioRxiv · Jun 18, 2020Biophysics

Democratising "Microscopi": a 3D printed automated XYZT fluorescence imaging system for teaching, outreach and fieldwork

M. B. Wincott, A. Jefferson, I. M. Dobbie, M. J. Booth, I. Davis, R. M. Parton

The paper describes a low-cost, 3D-printed automated fluorescence imaging system for teaching and outreach, demonstrating its multimodal imaging capabilities and open-source design.

bioRxiv · May 21, 2020Scientific Communication & Education

CryoSIM: super resolution 3D structured illumination cryogenic fluorescence microscopy for correlated ultra-structural imaging

M. A. Phillips, M. Harkiolaki, D. M. S. Pinto, R. M. Parton, A. Palanca, M. Garcia-Moreno, I. Kounatidis, J. Sedat, D. I. Stuart, A. Castello, M. J. Booth, I. Davis, I. M. Dobbie

The paper introduces cryoSIM, a super-resolution 3D fluorescence microscope for cryo-imaging that can be replicated with off-the-shelf components and open-source software.

bioRxiv · Mar 9, 2020Cell Biology

Correlative cryo-structured illumination fluorescence microscopy and soft X-ray tomography elucidates reovirus intracellular release pathway

M. Harkiolaki, I. Kounatidis, M. L. Stanifer, M. A. Phillips, P. Paul-Gilloteau, X. Helligenstein, H. Wang, C. A. Okolo, T. Fish, M. C. Spink, D. I. Stuart, I. Davis, S. Boulant, J. M. Grimes, I. M. Dobbie

This study develops a correlative imaging platform combining cryo-SIM and SXT to study reovirus release from intracellular vesicles, revealing novel virus-induced structures.

bioRxiv · Jan 13, 2020Cell Biology

BeamDelta: simple alignment tool for optical systems

N. J. Hall, D. M. S. Pinto, I. M. Dobbie

BeamDelta is an alignment tool for optical systems that provides a live view of laser beam positions to improve accuracy and reduce eye strain during alignment.

Wellcome Open Research · Dec 6, 2019Peer-reviewedBiophysics

IsoSense: frequency enhanced sensorless adaptive optics through structured illumination

M. Zurauskas, I. M. Dobbie, R. M. Parton, M. A. Phillips, A. Gohler, I. Davis, M. Booth

IsoSense improves sensorless adaptive optics by using structured illumination to enhance spatial frequencies for reliable wavefront sensing in microscopy.

bioRxiv · Jan 24, 2019Bioengineering