A. Castello

The PAZ domain of Aedes aegypti Dicer 2 is critical for accurate and high-fidelity size determination of virus-derived small interfering RNAs.

This study identifies the PAZ domain's role in ensuring accurate 21 nt vsiRNA production by Dcr2 in Aedes aegypti, critical for antiviral responses against Semliki Forest virus.

Copy number variation on ABCC2-DNMBP loci impacts the diversity and composition of the gut microbiota in pigs

This study identifies a copy number variant (CNV) in the ABCC2-DNMBP locus that influences gut microbiota diversity and composition in pigs.

Alphavirus infection triggers selective cytoplasmic translocation of nuclear RBPs with moonlighting antiviral roles

This study identifies nuclear RNA-binding proteins that are translocated to the cytoplasm during alphavirus infection and exhibit antiviral activity.

Absolute quantitation of individual SARS-CoV-2 RNA molecules: a new paradigm for infection dynamics and variant differences

The paper quantifies SARS-CoV-2 RNA molecules using smFISH, revealing genomic RNA stability, cell-to-cell replication variability, and slower replication kinetics for the B.1.1.7 variant compared to the Victoria strain.

Global analysis of protein-RNA interactions in SARS-CoV-2 infected cells reveals key regulators of infection

The paper identifies key host and viral RNA-binding proteins involved in SARS-CoV-2 infection and shows that drugs targeting these proteins can inhibit infection.

CryoSIM: super resolution 3D structured illumination cryogenic fluorescence microscopy for correlated ultra-structural imaging

The paper introduces cryoSIM, a super-resolution 3D fluorescence microscope for cryo-imaging that can be replicated with off-the-shelf components and open-source software.