K. Fujita

Raman microscopy of cryofixed biological specimens for high-resolution and high-sensitivity chemical imaging

This study improves Raman microscopy's signal-to-noise ratio and resolution by imaging cryofixed biological specimens, enabling high-content chemical imaging under physiological conditions.

Dynamic coordination of the lever-arm swing of human myosin II in thick filaments on actin

This study uses high-speed atomic force microscopy to observe myosin II lever-arm coordination in synthetic thick filaments, revealing how structural mismatches enhance contraction efficiency.

Phosphorylation-controlled cohesion of a nuclear condensate regulates mRNA retention

The paper shows that phosphorylation controls nuclear speckle cohesion, affecting mRNA retention and that environmental stress alters this process.

Time-deterministic cryo-optical microscopy

The paper introduces a technique to rapidly freeze biological systems during optical observation, enabling detailed time-specific analysis of cellular dynamics at high spatial resolution.

Selective plane activation structured illumination microscopy

This study improves structured illumination microscopy by using selective plane illumination and photoswitchable proteins to eliminate out-of-focus background, enabling high-resolution imaging of volumetric samples.

Combined loss of obscurin and obscurin-like 1 in murine hearts results in diastolic dysfunction, altered metabolism and deregulated mitophagy.

This study shows that loss of obscurin and Obsl1 in mice causes diastolic dysfunction, altered metabolism, and deregulated mitophagy in the heart.

Deep learning generates custom-made logistic regression models for explaining how breast cancer subtypes are classified

The paper develops an explainable deep learning model to generate logistic regression models for breast cancer subtype classification, showing its utility in identifying gene-subtype relationships.

Wavefront-sensorless adaptive optics with a laser-free spinning disk confocal microscope

The paper introduces wavefront-sensorless adaptive optics for a laser-free spinning disk confocal microscope, improving signal, contrast, and resolution by correcting specimen-induced aberrations.

Exploring rare cellular activity in more than one million cells by a trans-scale-scope

The paper introduces an imaging system to observe rare cellular activity in over one million cells and demonstrates its ability to detect rare events in biological systems.

Crystal structure of β-L-arabinobiosidase belonging to glycoside hydrolase family 121

The paper presents the crystal structure of β-L-arabinobiosidase (HypBA2) from Bifidobacterium longum, revealing its catalytic mechanism and conserved residues essential for substrate binding and catalysis.

Improved daylight vision following AAV-mediated expression of R9AP in murine rod photoreceptors

This study shows that over-expressing R9AP in rod photoreceptors of mice lacking cone function improves their ability to respond to bright light, suggesting potential for treating cone dysfunction in humans.

Single AAV-mediated mutation replacement genome editing in limited number of photoreceptors mediate marked visual restoration

The paper develops an AAV-based genome editing platform to replace mutations in retinal neurons, achieving significant visual restoration in blind mice.

Direct visualization of human myosin II force generation using DNA origami-based thick filaments

This study directly visualizes human myosin II force generation using DNA origami-based thick filaments and single-molecule imaging, revealing key dynamics of the lever-arm swing and actin interaction.

Two Novel α-L-Arabinofuranosidases from Bifidobacterium longum subsp. longum belonging to Glycoside Hydrolase Family 43 Cooperatively Degrade Arabinan

This paper identifies two novel α-L-arabinofuranosidases from Bifidobacterium longum subsp. longum that degrade arabinan by targeting different linkages.