Author

M. Harkiolaki

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

Click to filter author papers

Microbiology· 4 Cell Biology· 3

Signature Assays, Organisms & Methods

Click to filter · or open tag

Research Focus:cell type annotation· 4 Assay:imaging· 4 Approach:microscopy & imaging analysis· 4 Research Focus:cell signaling & trafficking· 3 Approach:experimental assay· 3 Assay:fluorescence / live-cell microscopy· 3 Organism:human· 3 Assay:cryo-EM· 2

Frequent Co-Authors

I. Kounatidis· 3 shared I. M. Dobbie· 3 shared K. L. Nahas· 3 shared S. C. Graham· 3 shared C. M. Crump· 2 shared D. I. Stuart· 2 shared I. Davis· 2 shared M. A. Phillips· 2 shared

Activity by Year

2020-2024 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 25 mutual co-author links

Click any collaborator node to inspect or filter shared papers

Focal author (center)Selected co-authorORCID co-authorUnlinked name

Curved chords = mutual co-authorship

3 shared papersCell Biology

I. Kounatidis

Mutual co-authors in cluster

Author profile →

7 papers

Recent·Most cited

Applying 3D correlative structured illumination microscopy and X-ray tomography to characterise herpes simplex virus-1 morphogenesis

K. L. Nahas, V. Connor, K. J. Wijesinghe, H. G. Barrow, I. M. Dobbie, M. Harkiolaki, S. C. Graham, C. M. Crump

This study uses 3D correlative fluorescence and X-ray tomography to analyze HSV-1 mutants, revealing roles of viral proteins in morphogenesis and showing envelopment occurs via budding.

bioRxiv · Mar 13, 2024Microbiology

Progression of herpesvirus infection remodels mitochondrial organization and metabolism

S. Leclerc, A. Gupta, V. Ruokolainen, J.-H. Chen, K. Kunnas, A. Ekman, H. Niskanen, I. Belevich, H. Vihinen, P. Turkki, A. Perez-Berna, S. Kapishnikov, E. Mäntylä, M. Harkiolaki, E. Dufour, , M. Vihinen-Ranta

This study shows how herpesvirus infection alters mitochondrial structure and metabolism during infection progression.

bioRxiv · Nov 16, 2023 · PreprintMicrobiology

Contour, a semi-automated segmentation and quantitation tool for cryo-soft-X-ray tomography

K. L. Nahas, J. Ferreira Fernandes, C. Crump, S. C. Graham, M. Harkiolaki

Contour is a semi-automated tool for segmenting and quantifying cryo-soft-X-ray tomography data, enabling faster and more accurate analysis of cellular compartments.

Biological Imaging · May 17, 2022Peer-reviewedCell Biology

Near-native state imaging by cryo-soft-X-ray tomography reveals remodelling of multiple cellular organelles during HSV-1 infection

K. L. Nahas, V. Connor, K. M. Scherer, C. F. Kaminski, M. Harkiolaki, C. M. Crump, S. C. Graham

This study uses cryo-soft-X-ray tomography to image HSV-1 infected cells in near-native state, revealing morphological changes in organelles like mitochondria and cytoplasmic vesicles.

bioRxiv · Oct 11, 2021Microbiology

SARS-CoV-2 Assembly and Egress Pathway Revealed by Correlative Multi-modal Multi-scale Cryo-imaging

L. Mendonca, A. Howe, J. B. Gilchrist, D. Sun, M. Knight, L. C. Zanetti-Domingues, B. Bateman, A.-S. Krebs, L. Chen, J. Radecke, Y. Sheng, V. D. Li, T. Ni, I. Kounatidis, M. A. Koronfel, , P. Zhang

This study uses cryo-imaging to reveal SARS-CoV-2 replication, assembly, and egress pathways in infected cells.

bioRxiv · Nov 5, 2020 · PreprintMicrobiology

CryoSIM: super resolution 3D structured illumination cryogenic fluorescence microscopy for correlated ultra-structural imaging

M. A. Phillips, M. Harkiolaki, D. M. S. Pinto, R. M. Parton, A. Palanca, M. Garcia-Moreno, I. Kounatidis, J. Sedat, D. I. Stuart, A. Castello, M. J. Booth, I. Davis, I. M. Dobbie

The paper introduces cryoSIM, a super-resolution 3D fluorescence microscope for cryo-imaging that can be replicated with off-the-shelf components and open-source software.

bioRxiv · Mar 9, 2020Cell Biology

Correlative cryo-structured illumination fluorescence microscopy and soft X-ray tomography elucidates reovirus intracellular release pathway

M. Harkiolaki, I. Kounatidis, M. L. Stanifer, M. A. Phillips, P. Paul-Gilloteau, X. Helligenstein, H. Wang, C. A. Okolo, T. Fish, M. C. Spink, D. I. Stuart, I. Davis, S. Boulant, J. M. Grimes, I. M. Dobbie

This study develops a correlative imaging platform combining cryo-SIM and SXT to study reovirus release from intracellular vesicles, revealing novel virus-induced structures.

bioRxiv · Jan 13, 2020Cell Biology