Author

S. C. Graham

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Microbiology· 11 Biochemistry· 6 Cell Biology· 2 Immunology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 16 Organism:human· 10 Research Focus:host-pathogen & immune response· 8 Research Focus:cell signaling & trafficking· 7 Assay:proteomics· 6 Research Focus:cell type annotation· 5 Research Focus:protein structure prediction· 4 Assay:imaging· 3

Frequent Co-Authors

J. E. Deane· 8 shared C. M. Crump· 6 shared V. Connor· 5 shared D. I. Svergun· 4 shared M. Shamin· 4 shared C. M. Jeffries· 3 shared E. Emmott· 3 shared K. L. Nahas· 3 shared

Activity by Year

2017-2024 · peak 4/yr

Co-Author Collaboration Network

10 top collaborators · 28 mutual co-author links

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8 shared papersMicrobiology

J. E. Deane

Mutual co-authors in cluster

Author profile →

20 papers

Recent·Most cited

Applying 3D correlative structured illumination microscopy and X-ray tomography to characterise herpes simplex virus-1 morphogenesis

K. L. Nahas, V. Connor, K. J. Wijesinghe, H. G. Barrow, I. M. Dobbie, M. Harkiolaki, S. C. Graham, C. M. Crump

This study uses 3D correlative fluorescence and X-ray tomography to analyze HSV-1 mutants, revealing roles of viral proteins in morphogenesis and showing envelopment occurs via budding.

bioRxiv · Mar 13, 2024Microbiology

Human cytomegalovirus deploys molecular mimicry to recruit VPS4A to sites of virus assembly

B. G. Butt, D. Fischer, A. R. Rep, M. Schauflinger, C. Read, T. Bock, M. Hirner, S. C. Graham, J. von Einem

This study identifies a viral motif in pUL71 that mimics cellular ESCRT-III components to recruit VPS4A to the HCMV assembly site, though its functional role remains unclear.

bioRxiv · Jan 4, 2024Cell Biology

Zika viruses encode multiple upstream open reading frames in the 5' viral region with a role in neurotropism

C. Lefevre, G. M. Cook, A. M. Dinan, S. Torii, H. Stewart, G. Gibbons, A. S. Nicholson, L. Echavarria-Consuegra, L. W. Meredith, V. Lulla, J. C. Kenyon, I. G. Goodfellow, J. E. Deane, S. C. Graham, A. Lakatos, , N. Irigoyen

This study identifies multiple upstream open reading frames (uORFs) in Zika virus (ZIKV) that influence neurotropism but not mosquito transmission.

bioRxiv · Apr 20, 2023 · PreprintMicrobiology

Attenuation hotspots in neurotropic human astroviruses

H. Ali, A. Lulla, A. S. Nicholson, E. B. Wignall-Fleming, R. L. OConnor, D.-L. Vu, S. C. Graham, J. E. Deane, S. Guix, V. Lulla

This study identifies attenuation hotspots in neurotropic human astroviruses by analyzing dispensable regions in the capsid-coding region and confirms their role in neuronal attenuation using recombinant viruses.

bioRxiv · Sep 6, 2022Microbiology

Determinants of receptor tyrosine phosphatase homophilic adhesion: structural comparison of PTPRK and PTPRM extracellular domains

I. M. Hay, M. Shamin, E. R. Caroe, A. S. A. Mohammed, D. I. Svergun, C. M. Jeffries, S. C. Graham, H. J. Sharpe, J. E. Deane

This study compares the structural features of PTPRK and PTPRM extracellular domains to identify determinants of homophilic adhesion, finding that shape complementarity and limited sequence differences drive specificity.

bioRxiv · Jun 23, 2022Biochemistry

Herpes simplex virus 1 protein pUL21 stimulates cellular ceramide transport by activating CERT

T. H. Benedyk, V. Connor, E. R. Caroe, M. Shamin, D. I. Svergun, J. E. Deane, C. M. Jeffries, C. M. Crump, S. C. Graham

This paper shows HSV-1 protein pUL21 activates CERT to increase ceramide to sphingomyelin conversion, but does not affect virus replication or spread.

bioRxiv · Jun 1, 2022Microbiology

Contour, a semi-automated segmentation and quantitation tool for cryo-soft-X-ray tomography

K. L. Nahas, J. Ferreira Fernandes, C. Crump, S. C. Graham, M. Harkiolaki

Contour is a semi-automated tool for segmenting and quantifying cryo-soft-X-ray tomography data, enabling faster and more accurate analysis of cellular compartments.

Biological Imaging · May 17, 2022Peer-reviewedCell Biology

The crystal structure of vaccinia virus protein E2 and perspectives on the prediction of novel viral protein folds

W. N. Gao, C. Gao, J. E. Deane, D. C. J. Carpentier, G. L. Smith, S. C. Graham

This paper reports the crystal structure of vaccinia virus protein E2 and demonstrates that AlphaFold2 accurately predicts its structure despite its novelty and lack of sequence homology.

bioRxiv · Oct 14, 2021Microbiology

Near-native state imaging by cryo-soft-X-ray tomography reveals remodelling of multiple cellular organelles during HSV-1 infection

K. L. Nahas, V. Connor, K. M. Scherer, C. F. Kaminski, M. Harkiolaki, C. M. Crump, S. C. Graham

This study uses cryo-soft-X-ray tomography to image HSV-1 infected cells in near-native state, revealing morphological changes in organelles like mitochondria and cytoplasmic vesicles.

bioRxiv · Oct 11, 2021Microbiology

A tetrameric assembly of saposin A: increasing structural diversity in lipid transfer proteins

M. Shamin, S. J. Spratley, S. C. Graham, J. E. Deane

The paper reports the crystal structure of a tetrameric assembly of murine saposin A, revealing structural diversity in lipid transfer proteins.

bioRxiv · Aug 26, 2021Biochemistry

pUL21 is a viral phosphatase adaptor that promotes herpes simplex virus replication and spread

T. H. Benedyk, J. Muenzner, V. Connor, Y. Han, K. Brown, K. J. Wijesinghe, Y. Zhuang, S. Colaco, G. A. Stoll, O. S. Tutt, S. Svobodova, D. I. Svergun, N. A. Bryant, J. E. Deane, A. E. Firth, , S. C. Graham

This study identifies pUL21 as a viral phosphatase adaptor that promotes HSV-1 replication and spread by directing dephosphorylation and antagonizing pUS3 kinase activity.

bioRxiv · Apr 13, 2021Microbiology

The SARS-CoV-2 Spike protein has a broad tropism for mammalian ACE2 proteins

C. Conceicao, N. Thakur, S. Human, J. T. Kelly, L. Logan, D. Bialy, S. Bhat, P. Stevenson-Leggett, A. Zagrajek, P. Hollinghurst, M. Varga, C. Tsirigoti, J. Hammond, H. J. Maier, E. Bickerton, , D. Bailey

This study shows SARS-CoV-2 Spike protein can bind to ACE2 receptors from multiple mammals, indicating broad host tropism and potential for zoonotic transmission.

bioRxiv · Jun 17, 2020Microbiology

Oropouche virus glycoprotein topology and cellular requirements for virus assembly

N. d. s. barbosa, J. O. Concha, L. L. daSilva, C. Crump, S. C. Graham

This study investigates the cellular requirements for Oropouche virus glycoprotein assembly and secretion, identifying the role of the ESCRT machinery and CHMP6 in virus release.

bioRxiv · May 28, 2020 · PreprintMicrobiology

Structure of herpes simplex virus pUL7:pUL51, a conserved complex required for efficient herpesvirus assembly

B. G. Butt, D. J. Owen, C. Jeffries, L. Ivanova, J. W. Houghton, M. F. Ahmed, R. Antrobus, D. I. Svergun, J. J. Welch, C. M. Crump, S. C. Graham

This paper solves the structure of the HSV-1 pUL7:pUL51 complex, showing its conservation across herpesviruses and its potential role in membrane remodeling during virus assembly.

bioRxiv · Oct 18, 2019Microbiology

Herpes simplex virus-1 pUL56 degrades GOPC to alter the plasma membrane proteome

T. K. Soh, C. T. R. Davies, J. Muenzner, V. Connor, C. R. Bouton, H. G. Barrow, C. Smith, E. Emmott, R. Antrobus, S. C. Graham, M. P. Weekes, C. M. Crump

This study identifies HSV-1 pUL56 as a protein that degrades GOPC, altering the plasma membrane proteome by affecting signaling receptors.

bioRxiv · Aug 8, 2019Microbiology

The lipid transfer protein Saposin B does not directly bind CD1d for lipid antigen loading

M. Shamin, T. H. Benedyk, S. C. Graham, J. E. Deane

This study investigates whether Saposin B directly binds CD1d for lipid antigen loading and finds no direct interaction.

bioRxiv · Jul 11, 2019Biochemistry

Molecular basis of Cul3 ubiquitin ligase subversion by vaccinia virus protein A55

C. Gao, M. A. Pallett, T. I. Croll, G. L. Smith, S. C. Graham

This study identifies the molecular mechanism by which vaccinia virus protein A55 subverts the Cul3 ubiquitin ligase, showing high-affinity binding and structural similarity to cellular BTB-Kelch proteins.

bioRxiv · Nov 5, 2018Biochemistry

IFIT3 and IFIT2/3 promote IFIT1-mediated translation inhibition by enhancing binding to non-self RNA.

R. C. Fleith, H. V. Mears, E. Emmott, S. C. Graham, D. S. Mansur, T. R. Sweeney

This study identifies how IFIT3 and IFIT2 enhance IFIT1's ability to inhibit translation by stabilizing its binding to non-self RNA.

bioRxiv · Feb 8, 2018Immunology

Proteomic and biochemical comparison of the cellular interaction partners of human VPS33A and VPS33B

M. R. Hunter, G. G. Hesketh, A.-C. Gingras, S. C. Graham

This study compares the interactomes of VPS33A and VPS33B using BioID, revealing distinct interaction partners and subcellular localization.

bioRxiv · Dec 20, 2017Biochemistry

VPS18 recruits VPS41 to the human HOPS complex via a RING-RING interaction

M. R. Hunter, E. J. Scourfield, E. Emmott, S. C. Graham

This study identifies a RING-RING interaction between VPS18 and VPS41 that recruits VPS41 to the human HOPS complex, showing this mechanism is not conserved in yeast.

Biochemical Journal · Oct 23, 2017Peer-reviewedBiochemistry