J. E. Chipuk

An optimized high-yield protocol for expression and purification of monomeric full-length BAX protein

The paper presents a high-yield protocol for expressing, purifying, and storing functional monomeric BAX protein, addressing challenges in its production and stability.

Sphingosine 1-phosphate receptor 1 inhibition induces a pro-apoptotic signaling cascade in T cells

This study shows that S1PR1 inhibition leads to T cell apoptosis by altering BCL2 family balance and JNK activity, with implications for immune responses in ulcerative colitis.

PPARdelta signaling activation improves metabolic and contractile maturation of human pluripotent stem cell-derived cardiomyocytes.

This study shows that activating PPARD signaling enhances metabolic and contractile maturation of human pluripotent stem cell-derived cardiomyocytes.

Repeated hypoglycemia blunts the responsiveness of glucose-inhibited GHRH neurons by remodeling neural inputs and disrupting mitochondrial structure and function.

This study shows repeated hypoglycemia reduces GHRH neuron responsiveness by altering neural inputs and mitochondrial function, suggesting mitochondrial dynamics as a target for preventing hypoglycemia unawareness.

Real-time integration of cell death mechanisms and proliferation kinetics at the single-cell and population-level using high-throughput live-cell imaging.

This paper introduces SPARKL, a method for analyzing cell death and proliferation using live-cell imaging, offering improved sensitivity and throughput compared to traditional methods.

Dietary intake regulates the circulating inflammatory monocyte pool

This study shows that fasting reduces circulating pro-inflammatory monocytes and their metabolic activity, while preserving their emergency mobilization during acute inflammation.