This study presents cryo-EM structures of the human TSEN*pre-tRNA complex, revealing its architecture and tRNA binding interfaces, and provides insights into PCH-causing mutations.

bioRxiv · Sep 2, 2022Biochemistry

Characterization of SARS2 Nsp15 Nuclease Activity Reveals it's Mad About U

M. N. Frazier, L. B. Dillard, J. M. Krahn, L. Perera, J. G. Williams, I. M. Wilson, Z. D. Stewart, M. C. Pillon, L. J. Deterding, M. J. Borgnia, R. E. Stanley

This study characterizes SARS2 Nsp15 nuclease activity, revealing its preference for purines 3 of cleaved uridines and its role in viral RNA processing.

bioRxiv · Jun 1, 2021Biochemistry

Cryo-EM Structures of the SARS-CoV-2 Endoribonuclease Nsp15

M. C. Pillon, M. N. Frazier, L. Dillard, J. G. Williams, S. Kocaman, J. M. Krahn, L. Perera, C. K. Hayne, J. Gordon, Z. D. Stewart, M. Sobhany, L. J. Deterding, A. L. Hsu, V. P. Dandey, M. J. Borgnia, R. E. Stanley

This study determines the cryo-EM structures of SARS-CoV-2 Nsp15, revealing its active site interactions with uridine and conformational changes, which inform potential therapeutic targets.

bioRxiv · Aug 11, 2020 · PreprintBiochemistry

Streptococcus pyogenes M1T1 variants activate caspase-1 and induce an inflammatory neutrophil phenotype

J. G. Williams, D. Ly, N. J. Geraghty, J. D. McArthur, H. Vyas, J. Gorman, J. A. Tsatsaronis, R. Sluyter, M. L. Sanderson-Smith

This study shows that M1T1 GAS variants induce caspase-1 activation and an inflammatory neutrophil phenotype, promoting inflammation but not bacterial clearance.

bioRxiv · Mar 2, 2020 · PreprintMicrobiology

Bacteria boost mammalian host NAD metabolism by engaging the deamidated biosynthesis pathway

I. Shats, J. Liu, J. G. Williams, L. J. Deterding, C. Lim, E. Lee, W. Fan, M. Sokolsky, A. V. Kabanov, J. Locasale, X. Li

This paper shows bacteria enhance mammalian NAD metabolism by using a deamidated biosynthesis pathway, contributing to drug resistance and improving NAD-boosting efficiency.

bioRxiv · Dec 7, 2018Biochemistry