M. J. Borgnia

Molecular basis of polyspecific drug binding and transport by OCT1 and OCT2

The paper presents cryo-EM structures of OCT1 and OCT2 in different states and combines them with functional experiments to reveal principles of organic cation recognition and the alternating access mechanism.

Structures of trehalose-6-phosphate synthase, Tps1, from the fungal pathogen Cryptococcus neoformans: a target for novel antifungals

This paper reports the cryo-EM structures of CnTps1 from Cryptococcus neoformans in unliganded and ligand-bound forms, revealing substrate-binding residues and tetramer stabilization, with implications for antifungal drug design.

Structural Basis for pre-tRNA Recognition and Processing by the Human tRNA Splicing Endonuclease Complex

This study presents cryo-EM structures of the human TSEN*pre-tRNA complex, revealing its architecture and tRNA binding interfaces, and provides insights into PCH-causing mutations.

Automated systematic evaluation of cryo-EM specimens with SmartScope

SmartScope automates cryo-EM specimen evaluation using deep learning, improving efficiency and reducing manual effort in structure determination.

Camel nanobodies broadly neutralize SARS-CoV-2 variants

The paper isolates two camel nanobodies that broadly neutralize SARS-CoV-2 and its variants, showing therapeutic potential in mice.

Untwisted α-synuclein Filaments formed in the Presence of Lipid Vesicles

This study investigates the structure of α-synuclein filaments formed in the presence of lipid vesicles, revealing untwisted structures not previously observed in vitro.

Characterization of SARS2 Nsp15 Nuclease Activity Reveals it's Mad About U

This study characterizes SARS2 Nsp15 nuclease activity, revealing its preference for purines 3 of cleaved uridines and its role in viral RNA processing.

Redox sensitive E2 Rad6 controls cellular response to oxidative stress via K63 ubiquitination of ribosomes.

The paper identifies Rad6 as a redox-sensitive E2 enzyme that modifies ribosomes with K63 ubiquitin during oxidative stress, linking it to translation control and stress response.

Distinct cryo-EM Structure of α-synuclein Filaments derived by Tau

This study reports a cryo-EM structure of α-synuclein filaments promoted by tau, revealing distinct conformations compared to in vitro filaments.

Beam image-shift accelerated data acquisition for near-atomic resolution single-particle cryo-electron tomography

This paper introduces BISECT, a method that accelerates cryo-electron tomography data acquisition and improves resolution by using beam-image shift and advanced processing techniques.

Cryo-EM Structures of the SARS-CoV-2 Endoribonuclease Nsp15

This study determines the cryo-EM structures of SARS-CoV-2 Nsp15, revealing its active site interactions with uridine and conformational changes, which inform potential therapeutic targets.

Fab-dimerized glycan-reactive antibodies neutralize HIV and are prevalent in humans and rhesus macaques

The paper identifies Fab-dimerized glycan-reactive antibodies in HIV-infected macaques and HIV-naïve humans that neutralize HIV-1 and can be elicited by vaccination.

Controlling the SARS-CoV-2 Spike Glycoprotein Conformation

The paper analyzes SARS-CoV-2 spike protein conformations and develops engineered constructs to control its mobility for vaccine design.

Disruption of the HIV-1 Envelope allosteric network blocks CD4-induced rearrangements

This study identifies a critical role for W571 in HIV-1 Env allostery by showing mutations disrupting CD4-induced rearrangements and rendering Env insensitive to CD4 binding.

Symmetry transitions during gating of the TRPV2 ion channel in lipid membranes

This study uses cryo-EM to show that RTx induces two-fold symmetric conformations of TRPV2, with greater symmetry in nanodiscs than in amphipol.

Structural basis for activation of Dot1L methyltransferase on the nucleosome by histone H2BK120 ubiquitylation

This study determines the cryo-EM structure of Dot1L bound to a ubiquitylated H2BK120 nucleosome, revealing how H2BK120 ubiquitylation activates Dot1L methylation of H3K79.