This paper determines the crystal structure of a broadly neutralizing antibody targeting the stem helix of coronaviruses and elucidates its mechanism of neutralization.

bioRxiv · Sep 14, 2022Immunology

Immunization with recombinant accessory protein-deficient SARS-CoV-2 protects against lethal challenge and viral transmission

C. Ye, J.-G. Park, K. Chiem, P. Dravid, A. Allue Guardia, A. Garcia-Vilanova, A. kapoor, M. R. Walter, J. Kobie, R. K. Plemper, J. B. Torrelles, L. Martinez-Sobrido

This study develops a live-attenuated SARS-CoV-2 vaccine by deleting two accessory proteins, showing it protects against viral challenge and transmission in animal models.

bioRxiv · Mar 13, 2022 · PreprintMicrobiology

A bifluorescent-based assay for the identification of neutralizing antibodies against SARS-CoV-2 variants of concern in vitro and in vivo

K. Chiem, D. M. Vasquez, J. Silvas, J.-G. Park, M. Piepenbrink, J. Sourimant, M. J. Lin, A. L. Greninger, R. K. Plemper, J. B. Torrelles, M. R. Walter, J. C. de la Torre, J. Kobie, L. Martinez-Sobrido

This paper develops a bifluorescent-based assay to identify neutralizing antibodies against SARS-CoV-2 variants in vitro and in vivo, showing it accurately reflects viral neutralization.

bioRxiv · Jun 28, 2021Microbiology

Visualization of SARS-CoV-2 infection dynamic

C. Ye, K. Chiem, J.-G. Park, J. Silvas, D. Morales Vasquez, J. B. Torrelles, J. Kobie, M. R. Walter, J. C. de la Torre, L. Martinez-Sobrido

This study develops a method to visualize SARS-CoV-2 infection dynamics using reporter-expressing recombinant viruses, enabling real-time tracking and antibody screening in vivo.

bioRxiv · Jun 3, 2021 · PreprintMicrobiology

Rapid in vitro assays for screening neutralizing antibodies and antivirals against SARS-CoV-2

J.-G. Oark, F. S. Oladduni, K. Chiem, C. Ye, M. Pipenbrink, T. Moran, M. Walter, J. Kobie, L. Martinez-Sobrido

This paper describes a rapid and reproducible plaque reduction microneutralization assay for identifying neutralizing antibodies and antivirals against SARS-CoV-2, suitable for high-throughput screening.

bioRxiv · Jul 22, 2020Microbiology

A broad and potent H1-specific human monoclonal antibody produced in plants prevents influenza virus infection and transmission in guinea pigs

J.-G. Park, C. Ye, M. Piepenbrink, A. Nogales, H. Wang, M. Shuen, A. Meyers, L. Martinez-Sobrido, J. Kobie

This paper produces a human monoclonal antibody against H1N1 influenza in plants and shows it prevents infection and transmission in guinea pigs, similar to mammalian-produced versions.

bioRxiv · Jan 13, 2020Immunology