This paper determines the crystal structure of a broadly neutralizing antibody targeting the stem helix of coronaviruses and elucidates its mechanism of neutralization.

bioRxiv · Sep 14, 2022Immunology

Immunization with recombinant accessory protein-deficient SARS-CoV-2 protects against lethal challenge and viral transmission

C. Ye, J.-G. Park, K. Chiem, P. Dravid, A. Allue Guardia, A. Garcia-Vilanova, A. kapoor, M. R. Walter, J. Kobie, R. K. Plemper, J. B. Torrelles, L. Martinez-Sobrido

This study develops a live-attenuated SARS-CoV-2 vaccine by deleting two accessory proteins, showing it protects against viral challenge and transmission in animal models.

bioRxiv · Mar 13, 2022 · PreprintMicrobiology

Potent universal-coronavirus therapeutic activity mediated by direct respiratory administration of a Spike S2 domain-specific human neutralizing monoclonal antibody

M. S. Piepenbrink, J.-G. Park, A. Deshpande, A. Loos, C. Ye, M. Basu, S. Sarkar, D. Chauvin, J. Woo, P. Lovalenti, N. B. Erdmann, P. A. Goepfert, V. L. Truong, R. A. Bowen, M. R. Walter, , J. J. Kobie

This study identifies a potent universal coronavirus neutralizing monoclonal antibody, 1249A8, effective against multiple variants and demonstrating therapeutic activity through intranasal administration.

bioRxiv · Mar 5, 2022 · PreprintImmunology

A bifluorescent-based assay for the identification of neutralizing antibodies against SARS-CoV-2 variants of concern in vitro and in vivo

K. Chiem, D. M. Vasquez, J. Silvas, J.-G. Park, M. Piepenbrink, J. Sourimant, M. J. Lin, A. L. Greninger, R. K. Plemper, J. B. Torrelles, M. R. Walter, J. C. de la Torre, J. Kobie, L. Martinez-Sobrido

This paper develops a bifluorescent-based assay to identify neutralizing antibodies against SARS-CoV-2 variants in vitro and in vivo, showing it accurately reflects viral neutralization.

bioRxiv · Jun 28, 2021Microbiology

Visualization of SARS-CoV-2 infection dynamic

C. Ye, K. Chiem, J.-G. Park, J. Silvas, D. Morales Vasquez, J. B. Torrelles, J. Kobie, M. R. Walter, J. C. de la Torre, L. Martinez-Sobrido

This study develops a method to visualize SARS-CoV-2 infection dynamics using reporter-expressing recombinant viruses, enabling real-time tracking and antibody screening in vivo.

bioRxiv · Jun 3, 2021 · PreprintMicrobiology

Epitope classification and RBD binding properties of neutralizing antibodies against SARS-CoV-2 variants of concern

A. Deshpande, B. D. Harris, L. Martinez-Sobrido, J. J. Kobie, M. R. Walter

This study classifies NAb epitopes against SARS-CoV-2 RBD and evaluates how variants affect NAb and ACE2 binding, identifying key mutations that impact neutralization and infectivity.

bioRxiv · Apr 13, 2021Immunology

Generation and Characterization of recombinant SARS-CoV-2 expressing reporter genes

K. Chiem, D. Morales Vasquez, J.-G. Park, R. N. Platt, T. Anderson, M. R. Walter, J. J. Kobie, C. Ye, L. Martinez-Sobrido

This paper generates and characterizes recombinant SARS-CoV-2 viruses expressing fluorescent or bioluminescent reporter genes, demonstrating their utility for tracking viral infection and screening therapeutics.

bioRxiv · Nov 16, 2020Microbiology

Therapeutic activity of an inhaled potent SARS-CoV-2 neutralizing human monoclonal antibody in hamsters

M. S. Piepenbrink, J.-G. Park, F. Oladunni, A. Deshpande, M. Basu, S. Sarkar, A. Loos, J. Woo, P. Lovalenti, D. Sloan, C. Ye, K. Chiem, N. B. Erdmann, P. A. Goepfert, V. L. Truong, , J. J. Kobie

This study evaluates an inhaled monoclonal antibody against SARS-CoV-2 in hamsters, showing reduced viral burden and lung pathology with low-dose inhalation.

bioRxiv · Oct 14, 2020Immunology