Author

J. M. Spraggins

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 7 Bioengineering· 1 Cancer Biology· 1 Cell Biology· 1 Microbiology· 1

Signature Assays, Organisms & Methods

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Research Focus:cell type annotation· 8 Approach:experimental assay· 8 Organism:human· 7 Assay:imaging· 7 Organism:mouse· 3 Assay:spatial transcriptomics· 3 Research Focus:differential expression· 2 Research Focus:host-pathogen & immune response· 2

Frequent Co-Authors

R. M. Caprioli· 7 shared E. K. Neumann· 4 shared N. H. Patterson· 4 shared A. B. Fogo· 3 shared C. J. Good· 3 shared E. S. Rivera· 3 shared J. L. Allen· 3 shared M. E. Colley· 3 shared

Activity by Year

2020-2024 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 35 mutual co-author links

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7 shared papersBiochemistry

R. M. Caprioli

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Author profile →

11 papers

Recent·Most cited

Mutant GNAS drives a pyloric metaplasia with tumor suppressive glycans in intraductal papillary mucinous neoplasia

V. Q.-H. Trinh, K. E. Ankenbauer, S. M. Torbit, J. Liu, M. Batardiere, B. Kumar, H. C. Maurer, F. Revetta, Z. Chen, A. Kruse, A. Judd, C. Copeland, J. Wong, O. Ben-Levy, B. Jarvis, , K. E. DelGiorno

This study identifies GNASR201C as a driver of pyloric metaplasia in IPMN, characterized by tumor-suppressive glycans that inhibit cancer progression.

bioRxiv · Feb 25, 2024 · PreprintCancer Biology

Revealing In Situ Molecular Profiles of Glomerular Cell Types and Substructures with Integrated Imaging Mass Spectrometry and Multiplexed Immunofluorescence Microscopy

A. B. Esselman, F. A. Moser, L. Tideman, L. G. Migas, K. V. Djambazova, M. E. Colley, E. L. Pingry, N. H. Patterson, M. A. Farrow, H. Yang, A. B. Fogo, M. de Caestecker, R. Van de Plas, J. M. Spraggins

This paper integrates MALDI IMS and MxIF microscopy to analyze lipid and molecular profiles of glomerular cell types and substructures in human kidney tissue.

bioRxiv · Feb 21, 2024 · PreprintBiochemistry

In situ lipidomics of Staphylococcus aureus osteomyelitis using imaging mass spectrometry

C. J. Good, C. E. Butrico, M. E. Colley, K. N. Gibson-Corley, J. E. Cassat, J. M. Spraggins, R. M. Caprioli

The paper uses imaging mass spectrometry to map lipid changes in S. aureus-infected murine femurs, identifying altered lipid species and their spatial distribution in abscesses.

bioRxiv · Dec 1, 2023 · PreprintBiochemistry

Characterizing Low-cost Registration for Photographic Images to Computed Tomography

M. E. Kim, H. H. Lee, K. Ramadass, C. Gao, K. Van Schaik, E. Tkaczyk, J. M. Spraggins, D. C. Moyer, B. A. Landman

The paper compares low-cost photographic imaging methods with a 3-D camera for registering photographic images to CT scans, finding iPhone imaging has lower landmark error in some cases.

bioRxiv · Sep 22, 2023 · PreprintBioengineering

A Lipid Atlas of the Human Kidney

M. A. Farrow, L. E. M. Tideman, E. K. Neumann, L. G. Migas, N. H. Patterson, M. E. Colley, J. L. Allen, E. L. Pingry, M. Dufresne, H. Yang, M. Brewer, E. S. Rivera, C. E. Romer, K. Djambazova, K. Sharman, , J. M. Spraggins

The paper constructs a spatio-molecular lipid atlas of the human kidney using multimodal imaging and machine learning, revealing lipid profile variations related to sex and BMI.

bioRxiv · Apr 7, 2022Biochemistry

Direct Imaging and Identification of Proteoforms up to 70 kDa from Human Tissue

P. Su, J. P. McGee, K. R. Durbin, M. A. R. Hollas, M. Yang, E. K. Neumann, J. L. Allen, B. S. Drown, F. A. Butun, J. B. Greer, B. P. Early, R. T. Fellers, J. M. Spraggins, J. Laskin, J. M. Camarillo, , N. L. Kelleher

This paper introduces PiMS, a method that detects proteoforms up to 70 kDa in human tissue with high spatial resolution, identifying key metabolic enzymes in kidney tissue.

bioRxiv · Dec 7, 2021 · PreprintBiochemistry

Multimodal Imaging Mass Spectrometry of Murine Gastrointestinal Tract with Retained Luminal Content Shows Molecular Localization Patterns

E. R. Guiberson, A. G. Wexler, C. J. Good, E. P. Skaar, J. M. Spraggins, R. M. Caprioli

This study uses multimodal imaging mass spectrometry to analyze murine gastrointestinal tract tissue and luminal content, revealing distinct molecular patterns during Clostridioides difficile infection.

bioRxiv · Oct 3, 2021Biochemistry

High Spatial Resolution MALDI Imaging Mass Spectrometry ofFresh-Frozen Bone

C. J. Good, E. K. Neumann, C. E. Butrico, J. E. Cassat, R. M. Caprioli, J. M. Spraggins

This study advances MALDI IMS techniques for fresh-frozen murine femurs, revealing lipid distributions linked to specific tissue structures and cell types.

bioRxiv · Oct 1, 2021Biochemistry

Highly Multiplexed Immunofluorescence of the Human Kidney using Co-Detection by Indexing (CODEX)

E. K. Neumann, E. S. Rivera, N. H. Patterson, J. L. Allen, M. Brewer, M. deCaestecker, A. B. Fogo, R. M. Caprioli, J. M. Spraggins

This paper applies CODEX multiplexed immunofluorescence to visualize 23 antigens in human kidney tissue, identifying major cell types and substructures with high resolution.

bioRxiv · Dec 4, 2020Cell Biology

Spatially-targeted proteomics of the host-pathogen interface during staphylococcal abscess formation

E. Guiberson, A. Weiss, D. J. Ryan, A. J. Monteith, K. Sharman, D. B. Gutierrez, W. J. Perry, R. M. Caprioli, E. P. Skaar, J. M. Spraggins

The paper uses spatially targeted proteomics to analyze host-pathogen interactions during staphylococcal abscess formation, identifying immune responses and bacterial contributions across different abscess regions.

bioRxiv · Sep 1, 2020Microbiology

Lipid Landscape of the Human Retina and Supporting Tissues Revealed by High Resolution Imaging Mass Spectrometry

D. M. Anderson, J. D. Messinger, N. H. Patterson, E. S. Rivera, A. Kotnala, J. M. Spraggins, R. M. Caprioli, C. A. Curcio, K. L. Schey

This study uses high-resolution imaging mass spectrometry to map lipid distributions in human retina and supporting tissues at laminar, cellular, and sub-cellular levels.

bioRxiv · Apr 8, 2020Biochemistry