J. Zimmerberg

Defining the EM-signature of successful cell-transfection

The paper uses electron microscopy to identify unique structural changes in cells after transfection with Lipofectamine 2000 and 3000, providing a method to distinguish successfully transfected cells.

Endothelial cells release microvesicles that harbor multivesicular bodies and secrete exosomes

The paper identifies a novel form of extracellular vesicles (MCMVs) released by endothelial cells that contain multivesicular bodies and release exosomes.

The Delta variant SARS-CoV-2 spike protein uniquely promotes aggregation of pseudotyped viral particles

This study shows that Delta variant SARS-CoV-2 spike proteins cause unique viral particle aggregation, which may affect infection kinetics.

PIEZO1-dependent erythrocyte dehydration as the mechanism for selection of an allele protecting from severe malaria.

The paper investigates how PIEZO1E756del variant causes RBC dehydration, inhibiting P. falciparum infection and protecting against severe malaria.

A high throughput screening assay for inhibitors of SARS-CoV-2 pseudotyped particle entry

This paper develops a high throughput screening assay using SARS-CoV-2 pseudotyped particles to identify inhibitors of viral entry, finding 7 active compounds effective against both pseudotyped particles and live virus.

Planar aggregation of the influenza viral fusion peptide alters membrane structure and hydration, promoting poration.

This study shows that influenza viral fusion peptides aggregate on membranes, altering structure and hydration to promote poration.

Identifying SARS-CoV-2 entry inhibitors through drug repurposing screens of SARS- S and MERS-S pseudotyped particles

This paper identifies six broad-spectrum inhibitors of SARS-CoV-2 and MERS-CoV spike-mediated cell entry through drug repurposing screens using pseudotyped particles.

Ultra-fast electroporation of giant unilamellar vesicles -- Experimental validation of a molecular model

The paper experimentally validates a molecular model of electroporation by comparing vesicle permeabilization kinetics with molecular simulations.

Contacting domains that segregate lipid from solute transporters in malaria parasites

The paper identifies membrane contact sites between the parasitophorous vacuolar membrane and parasite plasma membrane in malaria parasites, showing distinct localization of nutrient and lipid transporters.