M. Shen

Lipid Nanoparticle-Associated Inflammation is Triggered by Sensing of Endosomal Damage: Engineering Endosomal Escape without Side Effects

This paper identifies that lipid nanoparticle-induced inflammation arises from endosomal membrane damage and proposes strategies to mitigate it through galectin inhibition or ESCRT-recruiting lipids.

Efficient Generation of Human Pluripotent Stem Cells from Frozen Cord Tissue via Chemical Reprogramming

This study demonstrates an efficient chemical method to generate iPSCs from frozen umbilical cord tissue, highlighting its safety and potential for regenerative medicine.

Nanoporous PEGDA ink for High-Resolution Additive Manufacturing of Scaffolds for Organ-on-a-Chip

This paper develops a nanoporous PEGDA ink for high-resolution 3D printing of scaffolds in organ-on-a-chip systems, showing improved cell adhesion and viability.

Dearth of smoking-induced mutations in NSRO-driven non-small-cell lung cancer despite smoking exposure

The paper examines genomic and transcriptomic changes in NSRO-driven NSCLCs in smokers and non-smokers, finding no smoking-induced mutations but higher transcriptomic cell cycle activity in smokers.

Structural insights into Rad18 targeting by the SLF1 BRCT domains

The paper presents the crystal structure of SLF1tBRCT bound to Rad18 peptide, revealing mechanisms of Rad18 recognition and BRCT-mediated complex assembly.

High-Resolution Additive Manufacturing of a Biodegradable Elastomer with a Low-Cost LCD 3D Printer

This paper develops a VP-POMaC ink for high-resolution 3D printing using low-cost LCD printers, enabling biodegradable and elastomeric structures with tunable mechanical properties.

Digital Manufacturing of Functional Ready-to-Use Microfluidic Systems

This paper introduces a method to 3D print fully functional, self-contained microfluidic systems using digital manufacturing, eliminating the need for external peripherals.

Interior pH Sensing Residue of Human Voltage-Gated Proton Channel Hv1 is Histidine 168

This paper uses CpHMD simulations to identify H168 as an interior pH sensor in hHv1, supporting previous electrophysiological findings.

Single-cell Transcriptomic Analysis of Systemic Autoinflammatory Diseases with Anti-TNFα Therapy

This study uses single-cell RNA sequencing to analyze PBMCs and PMNs in SAIDs patients before and after anti-TNF therapy, revealing interferon responses in disease activity and unexpected cytokine elevation post-treatment.

An Integrative Approach to Dissect the Drug Resistance Mechanism of the H172Y Mutation of SARS-CoV-2 Main Protease

This study investigates how the H172Y mutation in SARS-CoV-2 Mpro reduces nirmatrelvir efficacy using simulations, AI, and experiments, revealing structural and functional changes in the enzyme.

A cocktail containing two synergetic antibodies broadly neutralizes SARS-CoV-2 and its variants including Omicron BA.1 and BA.2

This paper identifies a potent antibody cocktail that synergistically neutralizes SARS-CoV-2 and its variants, including Omicron BA.1 and BA.2.

Target Class Profiling of Small Molecule Methyltransferases

The paper develops a target class profiling approach to study small molecule methyltransferases, identifies a novel HNMT inhibitor, and demonstrates its utility for drug discovery.

A Suite of TMPRSS2 Assays for Screening Drug Repurposing Candidates as Potential Treatments of COVID-19

The paper develops and validates a series of assays to identify TMPRSS2 inhibitors as potential COVID-19 treatments, identifying six compounds with varying clinical statuses.

Discovery of lipid-mediated protein-protein interactions in living cells using metabolic labeling with photoactivatable clickable probes

The paper develops lipid PTM-specific probes to capture protein-protein interactions in living cells, identifying novel interactors of myristoylated proteins.

A high throughput screening assay for inhibitors of SARS-CoV-2 pseudotyped particle entry

This paper develops a high throughput screening assay using SARS-CoV-2 pseudotyped particles to identify inhibitors of viral entry, finding 7 active compounds effective against both pseudotyped particles and live virus.

DENV NS1 and MMP-9 cooperate to induce vascular leakage by altering endothelial cell adhesion and tight junction

This study identifies that DENV NS1 and MMP-9 cooperate to induce vascular leakage by altering endothelial cell adhesion and tight junctions, suggesting MMP-9 as a potential therapeutic target for severe dengue.

A hybrid in silico approach reveals novel inhibitors of multiple SARS-CoV-2 variants

The paper uses a hybrid in silico approach combining machine learning and pharmacophore modeling on NCATS screening data to identify novel inhibitors effective against multiple SARS-CoV-2 variants.

Ultrapotent SARS-CoV-2 neutralizing antibodies with protective efficacy against newly emerged mutational variants

This study identifies three ultrapotent SARS-CoV-2 neutralizing antibodies effective against B.1.351 variants, showing synergistic effects and protective efficacy in mice.

Characterization and tissue localization of zebrafish homologs of the human ABCB1 multidrug transporter

This study characterizes zebrafish homologs of the human ABCB1 gene, showing that abcb4 closely resembles P-gp in function and localization, while abcb5 differs.

Prediction of occult tumor progression via platelet RNAs in a mouse melanoma model: a potential new platform of cancer screening for early detection of cancer

This paper identifies platelet RNAs that predict occult tumor progression in a mouse melanoma model, showing high diagnostic accuracy for early cancer detection.

Spatiotemporal transcriptomic atlas of mouse organogenesis using DNA nanoball patterned arrays

This study develops Stereo-seq, a high-resolution spatial transcriptomic method, to analyze mouse organogenesis and map developmental disease loci.

Characterization and structural basis of a lethal mouse-adapted SARS-CoV-2

A mouse-adapted SARS-CoV-2 strain with RBD mutations caused 100% mortality in aged male BALB/c mice and showed altered receptor binding affinity.

Identification of Cross-Reactive CD8+ T Cell Receptors with High Functional Avidity to a SARS-CoV-2 Immunodominant Epitope and Its Natural Mutant Variants

The paper identifies cross-reactive CD8+ T cell receptors with high functional avidity to a SARS-CoV-2 epitope and its variants, showing their ability to activate and kill infected cells.

Modelling the epidemic trend of the 2019 novel coronavirus outbreak in China

The paper models the 2019-nCov epidemic in China, finding comparable transmissibility but lower fatality rates than SARS and MERS, and highlights the impact of early interventions.

A key linear epitope for a potent neutralizing antibody to SARS-CoV-2 S-RBD

This study identifies a linear epitope on SARS-CoV-2 S-RBD recognized by a potent neutralizing antibody and shows its potential for vaccine design.

Temporal Metabolic Characteristics and Transcriptomic Landscape of Islets and Liver Reveal Dynamic Pathophysiology and Interorgan Crosstalk in High-fat Diet-induced Diabetes

This study examines the temporal metabolic and transcriptomic changes in islets and liver of mice on high-fat diet, revealing dynamic pathophysiology and interorgan crosstalk in diabetes.

A rapid and efficient screening system for neutralizing antibodies and its application for the discovery of potent neutralizing antibodies to SARS-CoV-2 S-RBD

This paper introduces a rapid screening system to identify potent neutralizing antibodies against SARS-CoV-2 and demonstrates its effectiveness in discovering high-potency antibodies.

Drug Repurposing Screen for Compounds Inhibiting the Cytopathic Effect of SARS-CoV-2

The paper identifies 319 compounds with anti-SARS-CoV-2 activities through a high-throughput screen, including 91 approved drugs and 49 investigational drugs, with 230 compounds previously unreported.

Identification of SARS-CoV-2 3CL Protease Inhibitors by a Quantitative High-throughput Screening

This study identifies 23 SARS-CoV-2 3CLpro inhibitors through high-throughput screening, with several showing potent activity against viral infection.

Heparan sulfate assists SARS-CoV-2 in cell entry and can be targeted by approved drugs in vitro

This study shows heparan sulfate aids SARS-CoV-2 entry and identifies drugs that inhibit this process in vitro.