Jingheng Chen

A confined gene drive for population modification in the malaria vector Anopheles stephensi

The paper develops a confined gene drive system called TARE in Anopheles stephensi to modify mosquito populations, showing it can spread but faces challenges from fitness costs and resistance.

Assessing target genes for homing suppression gene drive

This study evaluates CRISPR-based homing gene drives targeting female fertility genes in Drosophila to suppress population growth, finding varying efficiency and fitness costs across targets.

Heterozygous variants in PLCG1 affect hearing, vision, cardiac, and immune function

The paper reports seven individuals with PLCG1 variants causing hearing, vision, cardiac, and immune issues, and shows these variants have gain-of-function effects in Drosophila models.

Improving the suppressive power of homing gene drive by co-targeting a distant-site female fertility gene

This paper introduces a two-target gene drive design that improves suppression efficiency by combining homing and fitness cost elements, showing success in Drosophila but highlighting remaining challenges.

Population suppression with dominant female-lethal alleles is boosted by homing gene drive

This study combines female-lethal alleles with homing gene drive to improve population suppression efficiency while maintaining self-limiting properties in mosquitoes.

Gene drive-based population suppression in the malaria vector Anopheles stephensi

This study develops a gene drive in Anopheles stephensi to suppress malaria populations by targeting the doublesex gene, showing partial success with resistance and improved conversion when combined with a vasa-Cas9 line.

A homing rescue gene drive with multiplexed gRNAs reaches high frequency in cage populations but generates functional resistance

This paper describes a homing gene drive targeting the hairy gene in insects, which spreads to high frequency in cage populations but generates functional resistance alleles despite using multiple gRNAs.

Full-length isoform sequencing for resolving the molecular basis of Charcot-Marie-Tooth 2A

The paper uses full-length isoform sequencing to identify a splicing defect in MFN2 causing CMT2A in a patient with undiagnosed neuropathy.

Assessment of distant-site rescue elements for CRISPR toxin-antidote gene drives

This paper evaluates distant-site rescue elements for CRISPR toxin-antidote gene drives and finds they fail for both haplolethal and haplosufficient target genes.