Juan Manuel Garcia-Arcos

Agile manufacturing of PDMS microfluidic devices for cell culture without a clean room

This paper describes a method to fabricate PDMS microfluidic devices using laser-cut master molds, enabling rapid and cost-effective prototyping without a clean room.

Analysis of 3D epithelial tissue packing reveals correlated patterns of cell height and skewing

This study analyzes 3D epithelial tissue packing in MDCK cells, revealing correlations between cell height, skewing, and substrate properties during proliferation.

Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM

Benchmarking fluorescence lifetime estimators for the membrane mechanosensor Flipper-TR across microscopes, laser frequencies, and photon budgets establishes practical guidelines for quantifying relative lipid packing dynamics in live cells.

Molecular basis for cellular compartmentalization by an ancient membrane fission mechanism

This study identifies an ancient membrane fission mechanism in Asgard archaea, showing ESCRT-III's role in cell compartmentalization predates eukaryogenesis.

Actin dynamics sustains spatial gradients of membrane tension in adherent cells

The paper demonstrates that adherent cells maintain spatial gradients of membrane tension through actin dynamics and membrane-cortex attachments, with tension lowest at adhesion edges and highest at protrusions.