Author

L. Giacani

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

Click to filter author papers

Microbiology· 9 Immunology· 1

Signature Assays, Organisms & Methods

Click to filter · or open tag

Approach:experimental assay· 8 Research Focus:cell type annotation· 4 Organism:human· 4 Research Focus:host-pathogen & immune response· 3 Assay:proteomics· 3 Approach:deep learning· 2 Research Focus:clustering· 1 Assay:DNA methylation· 1

Frequent Co-Authors

A. L. Greninger· 5 shared E. Romeis· 5 shared A. Haynes· 4 shared B. J. Molini· 4 shared N. A. P. Lieberman· 4 shared Q. Phung· 4 shared L. C. Tantalo· 3 shared A. Addetia· 2 shared

Activity by Year

2020-2024 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 39 mutual co-author links

Click any collaborator node to inspect or filter shared papers

Focal author (center)Selected co-authorORCID co-authorUnlinked name

Curved chords = mutual co-authorship

5 shared papersMicrobiology

A. L. Greninger

Mutual co-authors in cluster

Author profile →

10 papers

Recent·Most cited

A Novel Pan-Proteome Array for High-Throughput Profiling of the Humoral Response to Treponema pallidum subsp. pallidum: a Pre-Clinical Study

J. J. Campo, E. Romeis, A. Oberai, J. Pablo, C. Hung, A. Teng, A. D. Shandling, A. Phan, A. Haynes, L. Giacani

This study develops a pan-proteome array to profile the humoral response to Treponema pallidum in rabbits, identifying antigens for early diagnosis and vaccine development.

bioRxiv · Apr 20, 2024 · PreprintMicrobiology

Treponema pallidum periplasmic and membrane proteins are recognized by circulating and skin CD4+ T cells

T. B. Reid, C. Godornes, V. L. Campbell, K. J. Laing, L. C. Tantalo, A. Gomez, T. N. Pholsena, N. A. P. Lieberman, T. M. Krause, V. I. Cegielski, L. A. Culver, N. Nguyen, D. Q. Tong, K. L. Hawley, A. L. Greninger, , D. M. Koelle

The paper identifies Tp-specific CD4+ T cell responses in blood and skin of syphilis patients, showing persistence after treatment and highlighting potential vaccine targets.

bioRxiv · Feb 27, 2024Immunology

Treponema pallidum subsp. pallidum with an Artificially Impaired TprK Antigenic Variation System is Attenuated in the Rabbit Model of Syphilis

E. Romeis, N. A. P. Lieberman, B. J. Molini, L. C. Tantalo, B. Chung, Q. Phung, C. Avendano, A. Vorobieva, A. L. Greninger, L. Giacani

This study creates a T. pallidum strain with impaired TprK antigenic variation and shows it is attenuated in a rabbit syphilis model.

bioRxiv · Jan 18, 2023 · PreprintMicrobiology

In vitro Isolation of Treponema pallidum subsp. pallidum from Fresh and Frozen Needle Aspirates of Primary Experimental Syphilis Lesions

L. C. Tantalo, B. J. Molini, M. Bose, J. D. Klausner, L. Giacani

This paper describes an in vitro method to isolate Treponema pallidum subsp. pallidum from clinical samples without using rabbits.

bioRxiv · Sep 13, 2022Microbiology

High-throughput nanopore sequencing of Treponema pallidum tandem repeat genes arp and tp0470 reveals clade-specific patterns and recapitulates global whole genome phylogeny

N. A. P. Lieberman, T. D. Armstrong, B. Chung, D. Pfalmer, C. M. Hennelly, A. Haynes, E. Romeis, Q.-Q. Wang, R.-L. Zhang, C.-X. Kou, G. Ciccarese, I. Dal Conte, M. Cusini, F. Drago, S.-i. Nakayama, , A. L. Greninger

The paper uses nanopore sequencing to analyze repeat regions in T. pallidum genes, revealing clade-specific patterns that align with whole genome phylogeny.

bioRxiv · Aug 2, 2022Microbiology

B-cell epitope mapping of TprC and TprD variants of Treponema pallidum subspecies

B. J. Molini, M. Fernandez, C. Godornes, A. Vorobieva, S. Lukehart, L. Giacani

This study identifies B-cell epitopes in TprC and TprD variants of Treponema pallidum using pepscan mapping and antisera, showing cross-reactivity and surface-exposed loop targeting.

bioRxiv · Jan 26, 2022 · PreprintMicrobiology

Longitudinal accumulation of in vivo and in vitro-grown Treponema pallidum subsp. pallidum TprK variants in the presence and absence of immune pressure

M. J. Lin, A. Haynes, A. Addetia, N. A. P. Lieberman, Q. Phung, H. Xie, T. V. Nguyen, B. J. Molini, S. A. Lukehart, L. Giacani, A. L. Greninger

This study shows that T. pallidum generates TprK sequence diversity both under immune pressure and in vitro without immune selection, highlighting the role of gene conversion in antigenic variation.

bioRxiv · Jun 26, 2021Microbiology

Genetic Engineering of Treponema pallidum subsp. pallidum, the Syphilis Spirochete

E. Romeis, L. Tantalo, N. Lieberman, Q. Phung, A. Greninger, L. Giacani

This paper successfully genetically modifies Treponema pallidum by replacing a pseudogene with a kanamycin resistance cassette, enabling functional genetics studies of syphilis.

bioRxiv · May 7, 2021Microbiology

Estimation of Full-Length TprK Diversity in Treponema pallidum subspecies pallidum

A. Addetia, M. J. Lin, Q. Phung, H. Xie, M. Huang, G. Ciccarese, I. Dal Conte, M. Cusini, F. Drago, L. Giacani, A. L. Greninger

This study uses deep sequencing to characterize full-length TprK diversity in T. pallidum strains from human infections, revealing near-complete sequence divergence and estimating high antigenic variation potential.

mBio · Oct 27, 2020Peer-reviewedMicrobiology

Transcriptional and immunological analysis of the putative outer membrane protein and vaccine candidate TprL of Treponema pallidum

A. Haynes, M. Fernandez, E. Romeis, O. Mitja, K. Konda, S. Vargas, M. Eguiluz, C. Caceres, J. Klausner, L. Giacani

This paper investigates the transcriptional regulation of the TprL gene in Treponema pallidum, showing that phase variation through homopolymeric G sequences affects its expression and immune response.

bioRxiv · Sep 28, 2020Microbiology