High-plex spatial RNA imaging in one round with conventional microscopes using color-intensity barcodes
Tianyi Chang, Shihui Zhao, Kunyue Deng, Zhizhao Liao, Mingchuan Tang, Yanxi Zhu, Wuji Han, Chenxi Yu, Wenyi Fan, Mengcheng Jiang, Guanbo Wang, Dongfang Liu, Jirun Peng, Yuhong Pang, Peng Fei, , Yanyi Huang
This paper introduces PRISM, a method for high-plex spatial RNA imaging using color intensity barcodes, validated across multiple tissues and showing the role of CAFs in tumor microenvironments.
Nature Biotechnology · Oct 30, 2025Peer-reviewedBioengineering