Author

N. Hoppe

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

Click to filter author papers

Biochemistry· 5 Biophysics· 1

Signature Assays, Organisms & Methods

Click to filter · or open tag

Approach:experimental assay· 5 Assay:structural biology· 3 Organism:human· 2 Research Focus:protein folding & dynamics· 2 Research Focus:protein structure prediction· 2 Approach:biophysical modeling· 1 Research Focus:cell type annotation· 1 Research Focus:chromatin & epigenetic regulation· 1

Frequent Co-Authors

A. Manglik· 4 shared K. Zhang· 3 shared B. Faust· 2 shared I. Deshpande· 2 shared Y. Cheng· 2 shared A. A. Anand· 1 shared A. F. Brilot· 1 shared A. F. M. Pinto· 1 shared

Activity by Year

2020-2024 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 26 mutual co-author links

Click any collaborator node to inspect or filter shared papers

Focal author (center)Selected co-authorORCID co-authorUnlinked name

Curved chords = mutual co-authorship

4 shared papersBiochemistry

A. Manglik

Mutual co-authors in cluster

Author profile →

6 papers

Recent·Most cited

Molecular basis of proton-sensing by G protein-coupled receptors

M. K. Howard, N. Hoppe, X.-P. Huang, C. B. Macdonald, E. Mehrotra, P. Rockefeller Grimes, A. M. Zahm, D. D. Trinidad, J. G. English, W. Coyote-Maestas, A. Manglik

The paper identifies proton-sensing mechanisms in GPCRs using cryo-EM and deep mutational scanning, revealing a network of residues rather than a single site for proton recognition.

bioRxiv · Apr 17, 2024Biochemistry

GPR161 structure uncovers the redundant role of sterol-regulated ciliary cAMP signaling in the Hedgehog pathway

N. Hoppe, S. Harrison, S.-H. Hwang, Z. Chen, M. Karelina, I. Deshpande, C.-M. Suomivuori, V. R. Palicharla, S. P. Berry, P. Tschaikner, D. Regele, D. F. Covey, E. Stefan, D. S. Marks, J. Reiter, , A. Manglik

The paper determines the cryo-EM structure of active GPR161 bound to Gs, revealing sterol binding and its role in ciliary cAMP signaling, while showing that sterol binding is not essential for Hedgehog pathway suppression.

Nature Structural & Molecular Biology · Feb 7, 2024Peer-reviewedBiochemistry

Autoantibody and hormone activation of the thyrotropin G protein-coupled receptor

B. Faust, I. Singh, K. Zhang, N. Hoppe, A. F. M. Pinto, Y. Muftuoglu, C. B. Billesboelle, A. Saghatelian, Y. Cheng, A. Manglik

This study determines cryogenic-electron microscopy structures of active and inactive TSHR, revealing how autoantibodies and TSH activate the receptor through similar conformational changes.

bioRxiv · Jan 6, 2022 · PreprintBiochemistry

Fusion protein strategies for cryo-EM study of G protein-coupled receptors

K. Zhang, H. Wu, N. Hoppe, A. Manglik, Y. Cheng

The paper explores fusion protein strategies to improve cryo-EM structure determination of GPCRs without signaling proteins, achieving resolutions of 3.4 and 3.7 Å for two receptors.

bioRxiv · Dec 23, 2021Biophysics

Epistasis shapes the fitness landscape of an allosteric specificity switch

K. K. Nishikawa, N. Hoppe, R. Smith, C. Bingman, S. Raman

The paper investigates how epistasis influences the emergence of new inducer specificity in an allosteric transcription factor, revealing nonlinear relationships between local interactions and global function.

bioRxiv · Oct 21, 2020Biochemistry

An ultra-potent synthetic nanobody neutralizes SARS-CoV-2 by locking Spike into an inactive conformation

M. Schoof, B. Faust, R. A. Saunders, S. Sangwan, V. V. Rezelj, N. Hoppe, M. Boone, C. Billesboelle, C. Puchades, C. M. Azumaya, H. T. Kratochvil, M. Zimanyi, I. Deshpande, J. Liang, S. Dickinson, , Barile-H

This paper develops a potent synthetic nanobody that neutralizes SARS-CoV-2 by locking its Spike protein in an inactive conformation, enabling aerosol delivery as a prophylactic or therapeutic agent.

bioRxiv · Aug 8, 2020Biochemistry