Unambiguous detection of SARS-CoV-2 subgenomic mRNAs with single cell RNA sequencing
P. Cohen, E. J. DeGrace, O. Danziger, R. Patel, E. A. Barrall, T. Bobrowski, T. Kehrer, A. Cupic, L. Miorin, A. Garcia-Sastre, B. R. Rosenberg
This study evaluates scRNA-Seq methods for detecting SARS-CoV-2 subgenomic mRNAs, finding that 10X 5' with extended R1 sequencing best captures these viral RNAs at single-cell resolution.
bioRxiv · Nov 22, 2021Microbiology