Grant A. Rybnicky

Signal sequences target enzymes and structural proteins to bacterial microcompartments and are critical for microcompartment formation

This study investigates the role of signal sequences in bacterial microcompartment (MCP) assembly, showing that structural protein signal sequences are critical for MCP formation and that their deletion causes defects similar to full gene knockout.

Developing, characterizing and modelingCRISPR-based point-of-use pathogen diagnostics

The paper develops and optimizes a one-pot NASBA-Cas13a assay for rapid, sensitive detection of SARS-CoV-2 RNA without specialized equipment.

At-home, cell-free synthetic biology education modules for transcriptional regulation and environmental water quality monitoring

The paper develops four cell-free biosensing modules for synthetic biology education that enable students to explore transcriptional regulation and environmental monitoring without lab equipment.

Development of a Freeze-Dried CRISPR-Cas12 Sensor for Detecting Wolbachia in the Secondary Science Classroom

The paper develops a freeze-dried CRISPR-Cas12 sensor for detecting Wolbachia in arthropod samples, suitable for secondary education.

Spacer2PAM: A computational framework for identification of functional PAM sequences for endogenous CRISPR systems

Spacer2PAM predicts functional PAM sequences for endogenous CRISPR systems, enabling targeted genome modification in difficult-to-transform organisms.

Development of a clostridia-based cell-free system for prototyping genetic parts and metabolic pathways

This paper develops a high-yielding cell-free gene expression system from Clostridium autoethanogenum to enable in vitro prototyping of genetic parts and metabolic pathways.