S. Hohng

Mature microRNA-binding protein QKI promotes microRNA-mediated gene silencing

The paper identifies QKI as a novel microRNA-binding protein that enhances AGO2/let-7b-mediated gene silencing and suppresses tumor growth.

CHD7 interacts with the nucleosome acidic patch for its efficient activity via its N-terminal region

This study identifies the N-terminal region of CHD7 as critical for its ATPase activity and nucleosome remodeling through interactions with the nucleosome acidic patch.

Yeast Chd1p remodels nucleosomes with unique DNA unwrapping and translocation dynamics

This study uses single-molecule techniques to show that yeast CHD1 remodels nucleosomes through unique DNA unwrapping and translocation dynamics involving ATP binding and hydrolysis.

Transcription reinitiation by recycling RNA polymerase that diffuses on DNA after releasing terminated RNA

The paper uses single-molecule fluorescence to show that RNA release precedes RNA polymerase dissociation during bacterial intrinsic termination, revealing a recycling stage where RNAP diffuses and can reinitiate transcription.

Structural basis of recognition and destabilization of histone H2B ubiquitinated nucleosome by DOT1L histone H3 Lys79 methyltransferase

The paper presents cryo-EM structures of DOT1L with nucleosomes and shows H2B ubiquitination enhances DOT1L's non-catalytic nucleosome destabilization function.

Accelerated FRET-PAINT Microscopy

This paper improves FRET-PAINT microscopy speed by 8-fold through optimizations in camera speed, probe dissociation, and signal bleed-through, achieving 40-nm resolution in tens of seconds.