J. Y. Lee

Selective enrichment of Methylococcaceae versus Methylocystaceae methanotrophs via control of methane feeding scheme

This study enriches methanotrophic microbiomes from paddy soils under different methane and copper conditions, finding Methylocystis dominance and ecological importance of methanobactin in Cu-deficient environments.

Mammalian glial protrusion transcriptomes predict localization of Drosophila glial transcripts required for synaptic plasticity

The paper predicts Drosophila glial transcripts involved in synaptic plasticity by analyzing mammalian glial transcriptomes and validates their localization and functional importance.

Optimized path planning surpasses human efficiency in cryo-EM imaging

This paper introduces an algorithm called cryoRL that uses reinforcement learning to optimize cryo-EM data collection, outperforming human operators in efficiency and image quality.

Systematic analysis of YFP gene traps reveals common discordance between mRNA and protein across the nervous system

The paper systematically analyzes YFP gene traps in Drosophila to show widespread discordance between mRNA and protein expression in the nervous system, revealing common post-transcriptional regulation.

Clinical and immunological signatures of severe COVID-19 in previously healthy patients with clonal hematopoiesis

This study identifies clonal hematopoiesis as an independent risk factor for severe COVID-19 in previously healthy patients, revealing distinct immunological and epigenetic signatures.

Microbiota induces aging-related leaky gut and inflammation by dampening mucin barriers and butyrate-FFAR2/3 signaling

The paper shows that aged microbiota causes leaky gut and inflammation by reducing butyrate and FFAR2/3, which weakens mucin barriers.

A noble TGF beta biogenesis inhibitor exhibits both potent anti-fibrotic and anti-inflammatory capabilities

This paper identifies ONG41008 as a TGFβ biogenesis inhibitor with potent anti-fibrotic and anti-inflammatory effects in IPF and inflammatory models.

Absolute quantitation of individual SARS-CoV-2 RNA molecules: a new paradigm for infection dynamics and variant differences

The paper quantifies SARS-CoV-2 RNA molecules using smFISH, revealing genomic RNA stability, cell-to-cell replication variability, and slower replication kinetics for the B.1.1.7 variant compared to the Victoria strain.

An intranasal ASO therapeutic targeting SARS-CoV-2

This paper develops an intranasal ASO therapy targeting SARS-CoV-2 RNA that effectively prevents viral replication in multiple models and variants.

Strain variation in Bacillus cereus biofilms and their susceptibility to extracellular matrix-degrading enzymes

This study examines strain variation in Bacillus cereus biofilms and their susceptibility to extracellular matrix-degrading enzymes, revealing distinct structural and compositional differences among strains.

Validation of a Single-step, Single-tube Reverse Transcription-Loop-Mediated Isothermal Amplification Assay for Rapid Detection of SARS-CoV-2 RNA

The paper validates a single-step, single-tube RT-LAMP assay for rapid SARS-CoV-2 detection with high specificity and sensitivity.

Yeast Chd1p remodels nucleosomes with unique DNA unwrapping and translocation dynamics

This study uses single-molecule techniques to show that yeast CHD1 remodels nucleosomes through unique DNA unwrapping and translocation dynamics involving ATP binding and hydrolysis.

Transcription reinitiation by recycling RNA polymerase that diffuses on DNA after releasing terminated RNA

The paper uses single-molecule fluorescence to show that RNA release precedes RNA polymerase dissociation during bacterial intrinsic termination, revealing a recycling stage where RNAP diffuses and can reinitiate transcription.

Functional genomics reveals extensive diversity in Staphylococcus epidermidis restriction modification systems compared to Staphylococcus aureus.

The paper identifies extensive diversity in type I restriction modification systems in Staphylococcus epidermidis compared to Staphylococcus aureus, enabling efficient genetic manipulation of clinically relevant strains.

YAP-independent mechanotransduction drives breast cancer progression

This study shows that mechanotransduction in breast cancer occurs without YAP, due to compact nuclear morphologies in vivo.