S. M. Karst

High-throughput DNA extraction and cost-effective miniaturized metagenome and amplicon library preparation of soil samples for DNA sequencing

The paper benchmarks DNA extraction methods and miniaturizes library preparation for soil metagenomics, improving efficiency and reducing costs.

Oxford Nanopore R10.4 long-read sequencing enables near-perfect bacterial genomes from pure cultures and metagenomes without short-read or reference polishing

This study demonstrates that Oxford Nanopore R10.4 long-read sequencing can generate near-perfect microbial genomes from isolates or metagenomes without short-read or reference polishing.

Genome-scale CRISPR Screens Identify Host Factors that Promote Human Coronavirus Infection

Genome-wide CRISPR screens identified host factors that promote human coronavirus infection, revealing novel antiviral drug targets.

Multi-omics profiling of Earth's biomes reveals patterns of diversity and co-occurrence in microbial and metabolite composition across environments

The paper performs a multi-omics meta-analysis of microbial and metabolite data from diverse environments, revealing patterns of diversity and co-occurrence across biomes.

Human norovirus infection of primary B cells triggers immune activation in vitro

This study shows human norovirus infects primary B cells, induces functional changes, and the NS1 protein affects B cell proliferation and metabolism in vitro.

Connecting structure to function with the recovery of over 1000 high-quality activated sludge metagenome-assembled genomes encoding full-length rRNA genes using long-read sequencing

The paper uses long-read sequencing to recover over 1000 high-quality MAGs from wastewater treatment plants, enabling linkage of microbial community structure to function.

Exploring the upper pH limits of nitrite oxidation: diversity, ecophysiology, and adaptive traits of haloalkalitolerant Nitrospira

This study identifies haloalkalitolerant Nitrospira species in extreme pH lakes, revealing their diversity and adaptive traits for nitrite oxidation at high pH.

A Novel Small Animal Model of Norovirus Diarrhea

This study identifies a murine norovirus that causes self-resolving diarrhea in neonatal mice without systemic disease, mirroring human norovirus infection and establishing a new animal model.

Novel prosthecate bacteria from the candidate phylum Acetothermia revealed by culture-independent genomics and advanced microscopy

The paper identifies a novel Acetothermia bacterium from anaerobic digesters, characterizes its genome and metabolism, and proposes a role for its unique prosthecae in nutrient uptake.

Identifying the abundant and active microorganisms common to full scale anaerobic digesters

The paper identifies abundant and active microorganisms in full-scale anaerobic digesters by analyzing microbial communities over six years using 16S rRNA sequencing.