T. Nagai

Autonomous multicolor bioluminescence imaging in bacteria, mammalian, and plant hosts

The paper develops a multicolor auto-bioluminescence system for use in bacteria, mammalian, and plant hosts, enabling long-term imaging without external substrates.

Creating coveted bioluminescence colors for simultaneous myriad-color bioimaging

The paper introduces a method to expand color variation in bioluminescence by tuning dual-acceptor BRET, enabling up to 20 colors for simultaneous bioimaging.

Time-deterministic cryo-optical microscopy

The paper introduces a technique to rapidly freeze biological systems during optical observation, enabling detailed time-specific analysis of cellular dynamics at high spatial resolution.

Selective plane activation structured illumination microscopy

This study improves structured illumination microscopy by using selective plane illumination and photoswitchable proteins to eliminate out-of-focus background, enabling high-resolution imaging of volumetric samples.

Negative legacy effects of past forest use on plant diversity in semi-natural grasslands on ski slopes

This study shows that ski slopes with a history of forest use have lower plant diversity compared to those with a pasture history, due to environmental changes and seed dispersal limitations.

Isoform-specific mutation in Dystonin-b gene causes late-onset protein aggregate myopathy and cardiomyopathy

This study identifies a Dst-b mutation causing late-onset myopathy and cardiomyopathy in mice, revealing its critical role in striated muscle maintenance.

Exploring rare cellular activity in more than one million cells by a trans-scale-scope

The paper introduces an imaging system to observe rare cellular activity in over one million cells and demonstrates its ability to detect rare events in biological systems.

Biophysical properties of intermediate states of EMT outperform both epithelial and mesenchymal states.

The paper investigates the biophysical properties of intermediate EMT states and finds they exhibit distinct structural and mechanical features compared to epithelial and mesenchymal states, with implications for tumor-cell dissemination.

A spontaneously blinking fluorescent protein for simple single laser super-resolution live cell imaging

The paper introduces a fluorescent protein, SPOON, that spontaneously blinks in response to light and dehydration, enabling simple single-laser super-resolution imaging.

Concept and in-silico assessment of an algorithm for monitoring cytosolic fluorescent aggregates in cells.

The paper introduces an algorithm for semi-automatic detection and segmentation of spot-like structures in fluorescence images, demonstrating its robustness in simulated and experimental conditions.