Andrew D. Stephens

The paper shows that transcriptional activity drives chromatin motion, which causes nuclear blebbing, independent of nuclear mechanics or actin antagonism.

This study shows lamin B1 affects nuclear shape and integrity via chromatin stiffness, not lamin stiffness, using mechanical measurements and simulations.

This study shows CAMSAP2 and Kif2a regulate midzone microtubule dynamics and cell abscission in human cells through their opposing roles in microtubule organization.

The paper examines chromatin states and transcription dynamics in nuclear blebs, finding variable chromatin states but consistent enrichment of transcription initiation over elongation.

This study shows that nuclear and actin mechanics change from G1 to G2, leading to decreased nuclear stiffness and increased vulnerability to rupture.

The paper shows camptothecin suppresses nuclear blebbing by stalling RNA Pol II and increasing chromatin stiffness.

The paper measures lamin B and DNA damage in blebbed nuclei to determine the effect of nuclear rupture, finding lamin B loss is rupture-dependent while DNA damage is independent.

This study shows that CTCF is essential for pericentromere function, mitotic fidelity, and post-mitotic nuclear shape by disrupting mitotic spindle organization and increasing intercentromere distances.

The paper investigates how nuclear blebs alter chromatin packing domains, finding fragmentation of nanoscopic heterochromatin domains within blebs.

This study shows DNA damage reduces heterochromatin via ATM, leading to nuclear softening, blebbing, and rupture in MEF cells.

The paper compares lamin B and DNA levels in nuclear blebs across cell types and perturbations, finding DNA concentration is more consistently reduced than lamin B levels.

This study introduces liquid chromatin Hi-C to analyze chromatin interaction dynamics and finds that compartmentalization stability varies with chromatin domain type and fragment size.