Joshua L. C. Wong

This paper identifies PgaR as a positive regulator of the pgaABCD operon in Klebsiella pneumoniae, showing that genetic variation in this region leads to loss or hyper-production of PNAG, with distinct fitness consequences.

This study identifies two IncHI1 megaplasmids in Klebsiella species carrying blaIMP-1, showing transposable element-mediated mobilisation and conjugative transfer.

This study identifies how personalized gut microbiotas influence the competitiveness of antibiotic-resistant pathogens through carbohydrate competition and specific genetic mutations.

This study shows hypervirulent Klebsiella pneumoniae uses its capsule to prevent neutrophil activation, allowing bacterial survival despite immune presence.

The paper shows that pilin cyclisation and pilus formation are robust to amino acid substitutions, but electrostatic changes impair conjugation efficiency.

This paper investigates phage-pathogen interactions in vivo using Citrobacter rodentium and a novel lytic phage, showing stable coexistence without reducing bacterial burden or inflammation.

The paper introduces a platform using Citrobacter rodentium to generate antibodies against bacterial polysaccharides and proteins without requiring purified or synthetic antigens.

The paper evaluates MrkA expression variability in Klebsiella pneumoniae and shows that anti-MrkA mAb efficacy depends on antigen abundance.

This paper characterizes a Klebsiella pneumoniae isolate resistant to three beta-lactam/beta-lactamase inhibitor combinations, identifying genetic factors contributing to its resistance.

The paper reports the cryo-EM structure of the H-pilus, revealing its cyclic pilin subunit and explaining its role in conjugation.

The study identifies 12 accessory T3SS effectors in Citrobacter rodentium that influence infection outcomes without affecting virulence.

This study shows that the hypervirulent Klebsiella pneumoniae capsule masks O-antigen, preventing neutrophil recognition and killing, while O-antigen can also trigger neutrophil degranulation and NET formation.

The paper determines the cryo-EM structure of TraT proteins from plasmids and reveals their evolutionary history, showing independent acquisition by different plasmid backbones.

This paper shows that Klebsiella pneumoniae produces extracellular PNAG in vitro but switches to cell-associated PNAG during lung infection, highlighting its potential as a vaccine antigen.