Jonathan Michael Irish

This study compares methods for generating single-cell suspensions from mouse brain tissue using mass cytometry, finding that mechanical dissociation with enzymes yields viable cells while Percoll reduces viability, and cryopreservation preserves cell composition.

Velociraptor identifies and searches for novel cell types across platforms using hallmark feature sets, revealing new biological insights in glioblastoma and immunology.

The paper identifies a hypometabolic CD8+ T cell state, TEff-2, linked to respiratory failure and mortality in critical illness using single-cell RNA sequencing.

The paper screens a compound library to identify leukemia-selective chemotypes using single-cell profiling and finds that certain rocaglate derivatives selectively induce DNA damage in leukemia cells.

The paper identifies distinct type 1 immune networks associated with varying severities of restrictive lung disease following COVID-19.

This study identifies IL-8 as a key factor instructing macrophage identity in glioblastoma, showing IL-8 is necessary for generating CD32+ M_IL-8 macrophages in tumors contacting the lateral ventricle.

This study identifies the role of 4EBP1/2 dephosphorylation in inducing quiescence in embryonic neural stem cells, offering insights into adult brain development and potential therapeutic strategies.

CASSATT processes cycIHC data to align images, segment nuclei, and analyze spatial cell interactions and neighborhoods.

T-REX identifies rare and common immune cell changes in COVID-19, rhinovirus infection, and cancer therapy using unsupervised machine learning.