Rebecca A. Ihrie

Velociraptor identifies and searches for novel cell types across platforms using hallmark feature sets, revealing new biological insights in glioblastoma and immunology.

This study uses imaging mass cytometry to analyze changes in glioblastoma tumor microenvironment before and after treatment, finding increased normal brain cells and altered cellular interactions post-treatment.

The paper screens a compound library to identify leukemia-selective chemotypes using single-cell profiling and finds that certain rocaglate derivatives selectively induce DNA damage in leukemia cells.

The paper shows that chromatin accessibility and DNA demethylation occur at different times during enhancer regulation, with methylation states predicting chromatin dynamics using machine learning.

This study shows that TSC2 mutant cells exhibit early neurodevelopmental abnormalities and DNA methylation changes, suggesting early gene regulation disruption in TSC.

The paper develops a machine learning tool to identify balloon cells in TSC brain tissues using imaging mass cytometry and a custom antibody panel.

This study uses patient-derived neurovascular units to show that TSC2 mutations impair BBB function, which can be rescued by wild-type astrocytes or rapamycin treatment.

This study identifies IL-8 as a key factor instructing macrophage identity in glioblastoma, showing IL-8 is necessary for generating CD32+ M_IL-8 macrophages in tumors contacting the lateral ventricle.

This paper shows that Jedi-1-mediated phagocytosis by microglia in the V-SVZ promotes neurogenesis by suppressing inflammatory pathways, and its loss leads to neuroinflammation and reduced neural precursor proliferation.

This study identifies the role of 4EBP1/2 dephosphorylation in inducing quiescence in embryonic neural stem cells, offering insights into adult brain development and potential therapeutic strategies.

CASSATT processes cycIHC data to align images, segment nuclei, and analyze spatial cell interactions and neighborhoods.

The paper develops a 3D-printing protocol and open-source software for creating standardized head molds for stereotaxic injections in neonatal mice, enabling reproducible and rapid mold generation across labs.