Robert Ernst

Mammalian MemPrep establishes the lipid composition of ER membranes in HEK293T cells

This study characterizes the lipid composition of mammalian ER membranes using MemPrep, showing a common lipid profile across ER subdomains and linking lipid and protein biophysical properties.

Lipid remodelling enables adaptation to chronic hyperosmotic stress

The paper shows that chronic hyperosmotic stress in budding yeast leads to lipid droplet accumulation and membrane remodelling, which are essential for cell fitness.

Ubiquitin-dependent signal amplification in lipid saturation sensing

The paper investigates how Mga2 converts membrane physical cues into biochemical outputs via ubiquitylation, revealing a signal amplification mechanism involving E3 ligase autoubiquitylation.

Reversible tuning of membrane sterol levels by cyclodextrin in a dialysis setting

This paper demonstrates a method to reversibly adjust cholesterol levels in proteoliposomes using methyl-β-cyclodextrin in dialysis, showing reversible effects on membrane properties and protein behavior.

Pex30-dependent membrane contacts sites maintain ER lipid homeostasis

This study identifies Pex30's role in maintaining ER lipid homeostasis through membrane contact sites with peroxisomes and vacuoles in budding yeast.

Laboratory yeast strains are highly diverse in lipid metabolism

This study compares lipidomes of five yeast strains, finding significant differences in lipid classes and molecular species, with transcriptomic data suggesting post-transcriptional regulation.