Radek Šachl

Quantification of lipid sorting during clathrin-mediated endocytosis

This study quantifies lipid sorting during clathrin-mediated endocytosis and finds that lipid asymmetry in the plasma membrane explains observed partitioning, with minimal selectivity for cytoplasmic leaflet lipids compared to non-vesicular trafficking.

Nanoscale PI(4,5)P2 partitioning driven by FGF2 oligomerization triggers membrane pore formation

This paper shows FGF2 oligomerization drives PI(4,5)P2 partitioning and membrane pore formation, enabling FGF2 translocation across the plasma membrane.

Quantitative imaging of species-specific lipid transport in mammalian cells

The paper quantifies lipid transport between organelles in mammalian cells, revealing non-vesicular transport as a key mechanism for species-specific lipid sorting.

Particle Tracking for Quantized Intensities Accelerated with Parallelism

QTrack improves particle tracking by directly modeling quantized photon detections, achieving faster and more accurate results than traditional methods.

Revised diffusion law permits quantitative nanoscale characterization of membrane organization

The paper revises the diffusion law to quantify nanoscale membrane organization parameters using STED-FCS data, validating the Saffman-Delbruck assumption for lipid diffusion.

Tracking Without Linking or Localization: Single-Photon Tracking

This paper introduces SP2T, a method for tracking fast-diffusing particles using 1-bit image data, showing it outperforms traditional methods and enables faster, more accurate tracking.