This paper introduces a top-down mass spectrometry workflow with DMTm technology and a new software platform to achieve deeper proteoform sequencing and improved sequence coverage.

bioRxiv · Jun 3, 2026 · PreprintBioinformatics

Targeted Analysis of >1500 Plasma Proteoforms via Individual Ion Mass Spectrometry

Aniel Sánchez, Indira Plá, Katrina N. Peterson, Vincent White, Troy D. Fisher, Michael A. R. Hollas, Taojunfeng Su, Nhat Hoang Van Le, Claire Harrington, Paolo Cravedi, David N. Assis, Paola A. Barrios, Therese Elaine Banea, Daniela P. Ladner, Eleonora Forte, , Neil L. Kelleher

This paper introduces two plasma proteoform panels, PPA 526 and PPA 1514, using Individual Ion Mass Spectrometry to quantify proteoforms in human plasma with high reproducibility and disease discrimination capability.

bioRxiv · May 31, 2026 · PreprintBiochemistry

Top-Down Proteomics Identifies Plasma Proteoform Signatures of Liver Cirrhosis Progression

Eleonora Forte, Jes M. Sanders, Indira Plá, Kanchustambham Vijayalakshmi, Michael A. R. Hollas, Che‐Fan Huang, Aniel Sánchez, Katrina N. Peterson, Rafael Donadelli Melani, Alexander A. Huang, Praneet Polineni, Julianna M. Doll, Zachary C. Dietch, Neil L. Kelleher, Daniela P. Ladner

This study uses top-down proteomics to identify plasma proteoform signatures that distinguish stages of liver cirrhosis progression.

Molecular & Cellular Proteomics · Nov 8, 2024Peer-reviewedMolecular Biology

Deep Profiling of Plasma Proteoforms with Engineered Nanoparticles for Top-down Proteomics

Che‐Fan Huang, Michael A. R. Hollas, Aniel Sánchez, Mrittika Bhattacharya, Giang Ho, Ambika Sundaresan, Michael A. Caldwell, Xiaoyan Zhao, Ryan W. Benz, Asim S. Siddiqui, Neil L. Kelleher

This study uses engineered nanoparticles to enrich low-abundant proteins in human plasma, enabling top-down proteomics to detect proteoforms at lower concentrations and expand proteome depth.

Journal of Proteome Research · Sep 23, 2024Peer-reviewedBiochemistry