Clara Aicart-Ramos

Structural elements required for the efficient loading and activation of HELB on RPA-coated single-stranded DNA

This paper identifies two structural elements in HELB essential for its interaction with RPA-coated ssDNA, showing how they facilitate RPA displacement and DNA loop formation.

FBH1 Reverses Stalled Replication Forks via Sequential Unwinding of Nascent Strands

This study shows FBH1 reverses replication forks through sequential unwinding of nascent strands without forming four-way junctions, distinct from other reversal factors.

OT-Curtains: an approach for studying protein interactions with DNA-ends using optical tweezers and confocal fluorescence microscopy

OT-Curtains enables real-time observation of DNA-end interactions using optical tweezers and microscopy, demonstrating applications in DNA repair and telomere biology.

Structural basis for fork reversal and RAD51 regulation by the SCF ubiquitin ligase complex of F-box helicase 1

This study reveals how SCFFBH1 promotes fork reversal and regulates RAD51 through DNA binding and ubiquitination, using cryo-EM structures and in vitro experiments.

RPA directly stimulates Mer3/HFM1 helicase processivity to ensure normal crossover formation in meiosis

This study identifies RPA as a direct interactor of Mer3/HFM1 helicase, showing its role in enhancing helicase processivity and crossover formation during meiosis.

Assembly of a segrosome by a CTP-independent ParB-like protein

This study identifies a CTP-independent ParABS system in Streptomyces coelicolor that segregates plasmid SCP2 through a ParT protein that diffuses on DNA without CTP binding or hydrolysis.

Human HELB is a processive motor protein which catalyses RPA clearance from single-stranded DNA

This study characterizes human HELB as a monomeric helicase that binds ssDNA, hydrolyzes ATP, and clears RPA from ssDNA through translocation.