Jonathan V. Rocheleau

Apollo-IRE1 is a genetically encoded sensor that detects ER stress by measuring IRE1 oligomerization and fluorescence anisotropy changes, enabling multiplexed imaging in living cells.

The paper develops an on-chip fluorescence anisotropy immunoassay for human C-peptide by optimizing antibody-tracer pairs to achieve high sensitivity and fast kinetics for microfluidic measurement.

This study shows that free cholesterol accumulation in macrophages activates a PARP1 response to DNA damage, which suppresses lipopolysaccharide-induced inflammation through a non-enzymatic mechanism.

This paper shows nicotinamide promotes pancreatic endocrine progenitor differentiation via PARP inhibition, increasing Nkx6.1 and NAD+/NADH ratio.