Author

Wolfgang Guba

F. Hoffmann-La Roche AGORCID 0000-0002-3285-5957Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Cell Biology· 1 Neuroscience· 1 Pharmacology & Toxicology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 3 Organism:human· 2 Research Focus:host-pathogen & immune response· 1 Assay:protein structure· 1 Research Focus:protein structure prediction· 1 Approach:statistical model· 1

Frequent Co-Authors

Arne Christian RuferiD· 2 shared David A. SykesiD· 2 shared Dmitry B. VeprintseviD· 2 shared Eline J. KoersiD· 2 shared Uwe GretheriD· 2 shared Adrián Morales-Pastor· 1 shared Azadeh ShahsavariD· 1 shared Bianca PlouffeiD· 1 shared

Activity by Year

2021-2025 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 33 mutual co-author links

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2 shared papersCell Biology

Arne Christian RuferORCID

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Author profile →

3 papers

Recent·Most cited

Multiple triggers converge to preferential effector coupling in the CB2R through a complex allosteric communication network

Adrián Morales-Pastor, Tamara Miljuš, Miguel Dieguez-Eceolaza, Tomasz Maciej Stępniewski, Vicente Ledesma-Martin, Franziska M. Heydenreich, Tilman Flock, Bianca Plouffe, Christian Le Gouill, Jean Duchaine, David A. Sykes, Colin Nicholson, Eline J. Koers, Wolfgang Guba, Arne Christian Rufer, , Jana Selent

The paper investigates how multiple triggers on an allosteric communication network influence preferential coupling of the CB2R to Gi2 over β-arrestin1, revealing insights into GPCR signaling mechanisms.

Nature Communications · Jun 11, 2025Peer-reviewedCell Biology

A universal cannabinoid CB1 and CB2 receptor TR-FRET kinetic ligand binding assay.

Leire Borrega Roman, Bradley L. Hoare, Miroslav Kosar, Roman C. Sarott, Kacper J. Patej, Jara Bouma, Morgan Scott-Dennis, Eline J. Koers, Thais Gazzi, Leonard Mach, Sergio Barrondo, Joan Sallés, Wolfgang Guba, Eric A. Kusznir, Marc Nazaré, , Dmitry B. Veprintsev

This paper develops a TR-FRET-based assay to measure ligand binding kinetics for CB1R and CB2R, finding that D77 enables accurate determination of on- and off-rates for various agonists and antagonists.

Frontiers in Pharmacology · Apr 9, 2025Peer-reviewedPharmacology & Toxicology

Structural insights into glycine reuptake inhibition

Azadeh Shahsavar, Peter Stohler, Gleb P. Bourenkov, Iwan Zimmermann, Martin Siegrist, Wolfgang Guba, Emmanuel Pinard, Steffen Sinning, Markus A. Seeger, Thomas R. Schneider, Roger J. P. Dawson, Poul Nissen

The paper determines the structure of GlyT1 bound to an inhibitor, revealing its mechanism of non-competitive inhibition and informing drug design.

Nature · Mar 3, 2021Peer-reviewedNeuroscience