Joshua J. Ziarek

A bivalent lysine-acetylated small-molecule binding site in MYC

This study identifies a bivalent binding site in MYC for small-molecule inhibitors and shows acetylation enhances inhibitor binding and oncogenic activity.

Differentiating 5-thiooxazoles from oxazolone-coupled thioamides in RiPP natural products

The paper differentiates 5-thiooxazoles from oxazolone-coupled thioamides in RiPP natural products using chemical and structural analysis of oxazolin and methanobactin.

Bis-hydroxylation of Homocitrulline Catalyzed by a Multinuclear Nonheme Iron-Dependent Oxidative Enzyme during RiPP Biosynthesis

The paper identifies a novel RiPP biosynthetic pathway in Streptomyces thermodiastaticus involving a YcaO-TfuA pair, a carbamoyltransferase, and a MNIO enzyme that bis-hydroxylates homocitrulline.

Intrinsic conformational equilibria position arrestin-2 for activation

This study identifies two distinct conformational equilibria in arrestin-2 that underlie its pre-activation state in solution.

Stabilization versus flexibility: detergent-dependent trade-offs in neurotensin receptor 1 GPCR ensembles

This study investigates how different detergents affect the stability and ligand binding of neurotensin receptor 1, revealing a trade-off between structural stabilization and conformational flexibility.

Mapping the Ligand-dependent Remodeling of the Conformational Entropy Landscape in Neurotensin Receptor 1 by NMR-guided Molecular Simulations

This study uses NMR and MD simulations to analyze conformational entropy changes in GPCRs, showing how dynamic fluctuations influence allosteric signaling and receptor function.

The isolated Stachel peptide of the adhesion G protein-coupled receptor GPR126 is intrinsically disordered

The study shows the ADGRG6/GPR126 Stachel peptide is intrinsically disordered in solution, with limited helical conformations detected by biophysical experiments.

Chemical control of CSA geometry enables relaxation-optimized 19F-13C NMR probes

This paper demonstrates how controlling the CSA tensor geometry in 19F-13C NMR probes can suppress transverse relaxation, enabling optimized probes for large biomolecular systems.

Dynamic hotspots in the Uba7 UFD direct UbcH8 recognition

This paper presents the NMR structure and functional analysis of the Uba7 UFD domain and its interaction with UbcH8, revealing structural flexibility and key residues critical for E2 recognition in the ISGylation pathway.

Insights on the GPCR helix 8 solution structure and orientation using a neurotensin receptor 1 peptide

This study investigates the structural stability and orientation of the neurotensin receptor 1 H8-Ctail using NMR in detergent and lipid environments, revealing its sensitivity to membrane conditions.

Expanding the genotype and phenotype spectrum of SYT1-associated neurodevelopmental disorder

This study expands the known genotype and phenotype spectrum of SYT1-associated neurodevelopmental disorder by analyzing 22 individuals with de novo missense variants.