Meindert Hugo Lamers

Cryo-EM structure of MutL-activated MutH

The paper presents the cryo-EM structure of MutL-bound MutH on a hemi-methylated GATC site, revealing the mechanism of MutH activation for strand discrimination.

Identification of determinants of high-fidelity DNA synthesis in M. smegmatis DnaE1 through in silico and in vivo approaches

The paper identifies amino acid changes in M. smegmatis DnaE1 that affect DNA polymerase fidelity through in silico and experimental approaches.

PyVADesign: a Python-based cloning tool for one-step generation of large mutant libraries

PyVADesign is a Python tool that streamlines the design and ordering of dsDNA fragments for generating large mutant libraries, with automated primer design.

DNA polymerase actively and sequentially displaces single-stranded DNA-binding proteins

This study investigates how DNA polymerase displaces SSBs during replication, showing sequential displacement and the role of SSB saturation in replication efficiency.

Promiscuous NAD-dependent dehydrogenases enable efficient bacterial growth on the PET monomer ethylene glycol

This study identifies NAD-dependent dehydrogenases in Paracoccus denitrificans that enable efficient bacterial growth on ethylene glycol, with implications for biotechnological applications.

Structural and functional analysis of the Mycobacterium tuberculosis MmpS5L5 efflux pump presages a pathway to increased bedaquiline resistance

This study determines the cryo-EM structure of the MmpS5L5 efflux pump in Mycobacterium tuberculosis and identifies mutations that enhance bedaquiline efflux, suggesting new resistance mechanisms.

Longitudinal cell division is associated with single mutations in the FtsZ-recruiting SsgB in Streptomyces

The paper shows that mutations in SsgB, a protein that recruits FtsZ, cause longitudinal cell division in Streptomyces by altering septum placement.