Tanmay A. M. Bharat

The life cycle of an archaeon with multiple membranes

This study reveals that Ignicoccus hospitalis uses a contractile CdvA-ESCRT-III ring to constrict its inner membrane, leading to multi-cytoplasmic clusters and subsequent release of progeny cells through outer membrane bursting.

Molecular architecture of colossal surface layers from hyperthermophilic archaea

This study determines the structure of a large S-layer protein from Pyrobaculum arsenaticum using cryo-EM and AlphaFold, revealing its unique multidomain architecture and evolutionary conservation.

Structural basis of biofilm formation mediated by the Pseudomonas aeruginosa fibrillar adhesin CdrA

This study determines the structure of CdrA, a fibrillar adhesin in Pseudomonas aeruginosa, revealing its role in biofilm formation and identifying targets for inhibition.

Intracellular barriers and receptor masking limit success of a Pseudomonas aeruginosa clinical phage family

The paper identifies surface receptor masking and internal defenses as barriers to phage therapy in Pseudomonas aeruginosa clinical isolates and presents strategies to overcome these barriers through phage engineering.

Oxygen gradients reshape cross-feeding through emergent spatial organization of gut commensal bacteria

The paper shows how oxygen gradients in the gut reshape cross-feeding between Bacteroides thetaiotaomicron and Escherichia coli through spatial organization and metabolic feedback.

Asgard archaeal origin of microtubules

The paper identifies tubulins in Asgard archaea and shows they form mini-microtubules with eukaryotic-like properties, suggesting an archaeal origin for microtubules.

Morphological transformation in Helicobacter pylori is a dynamic process leading to two types of coccoid

This paper investigates the morphological transformation of Helicobacter pylori from helical to coccoid form using microscopy techniques, revealing it occurs without a U-shaped intermediate and involves peptidoglycan thinning and membrane bulging.

An Asgard archaeon with internal membrane compartments

This paper identifies an Asgard archaeon with an endomembrane system that changes with cell state, challenging previous views on eukaryogenesis.

Sub-cellular chemical mapping in bacteria using correlated cryogenic electron and mass spectrometry imaging

This paper combines cryo-EM and FIB-SIMS to map sub-cellular chemical distributions in bacteria, revealing pollutant storage in phase-separated aggregates.

Targeted disruption of phage liquid crystalline droplets abolishes antibiotic tolerance of bacterial biofilms

This study shows that disrupting phage liquid crystalline droplets in Pseudomonas aeruginosa biofilms eliminates antibiotic tolerance by promoting antibiotic diffusion.

Extensive PFAS accumulation by human gut bacteria

This study shows human gut bacteria can accumulate PFAS, with some species like Bacteroides uniformis accumulating them to high concentrations, and identifies mechanisms involving transmembrane transport and efflux pumps.

Structural and functional analysis of the Mycobacterium tuberculosis MmpS5L5 efflux pump presages a pathway to increased bedaquiline resistance

This study determines the cryo-EM structure of the MmpS5L5 efflux pump in Mycobacterium tuberculosis and identifies mutations that enhance bedaquiline efflux, suggesting new resistance mechanisms.

Structure of the virulence-associated Neisseria meningitidis filamentous bacteriophage MDA{varphi}

This study presents the structure of the Neisseria meningitidis filamentous bacteriophage MDA{Phi}, revealing its organization and molecular mechanisms of biofilm formation and infection.

Mapping the ultrastructural topology of the corynebacterial cell surface

This study maps the ultrastructural topology of the corynebacterial cell surface using electron cryotomography and single particle cryomicroscopy, revealing the S-layer composition and its evolutionary uniqueness.

Tissue-Like Multicellular Development Triggered by Mechanical Compression in Archaea

This study shows mechanical compression induces tissue-like multicellularity in haloarchaea, forming structures with distinct molecular and mechanical features similar to eukaryotic tissues.

The mechanics of a continuous self-assembling surface-layer aids cell division in an archaeon

The paper shows that the S-layer in Sulfolobus acidocaldarius aids cell division by reshaping the cytokinetic furrow through its flexible self-assembly, enhancing ESCRT-III-dependent division under mechanical stress.

Structure of the Pseudomonas aeruginosa PAO1 Type IV pilus

This study reports the 3.2 Å resolution structure of the Pseudomonas aeruginosa PAO1 Type IV pilus using cryo-EM, revealing its architecture and potential functional roles.

An image processing pipeline for electron cryo-tomography in RELION-5

The paper introduces an image processing pipeline for electron cryo-tomography in RELION-5, enabling automated atomic model building from tomographic data.

Punctuated and continuous structural diversity of S-layers across the prokaryotic tree of life

This study elucidates the structural diversity of S-layers across prokaryotes using AlphaFold2multimer and electron cryomicroscopy, revealing evolutionary patterns and cell anchoring mechanisms.

Mass-selective and ice-free cryo-EM protein sample preparation via native electrospray ion-beam deposition

This paper introduces native ES-IBD for preparing high-purity, ice-free cryo-EM samples, showing structural integrity but limitations in 2D classes and 3D EM density maps.