Guillaume Romet‐Lemonne

Tropomodulin and Leiomodin Differentially Nucleate Tropomyosin Assembly on Actin Filaments

The paper shows tropomodulin and leiomodin nucleate tropomyosin clusters on actin filaments, with tropomodulin acting at the pointed end and leiomodin along the entire filament.

SPIN90 modulates the architecture of lamellipodial actin in an ARPC5L dependent fashion.

SPIN90 selectively activates ArpC5L-containing Arp2/3 complexes to make linear actin filaments, shaping lamellipodial filament orientation and protrusion efficiency in B16 melanoma cells.

Action of cofilin on ADP-Pi-containing actin filaments

This study investigates how cofilin binds and severs actin filaments containing ADP-Pi-actin, revealing that cofilin clusters form preferentially on ADP-actin subunits and severing is slower at ADP-Pi-actin boundaries.

Myosin-X sorts actin filaments into parallel bundles and tunes barbed-end dynamics

This study shows myosin-X can bundle actin filaments and regulate barbed-end dynamics, suggesting it can initiate filopodia without passive crosslinkers.

Vimentin promotes actin assembly by stabilizing ATP-actin subunits at the barbed end

This study shows vimentin promotes actin elongation by stabilizing ATP-actin subunits at the barbed end, and it enhances actin nucleation through a direct interaction with ATP-actin monomers.

Rapid aging and disassembly of actin filaments from two evolutionary distant yeasts

This study compares actin filament assembly and disassembly kinetics between two yeast species and mammalian actin, revealing faster aging in yeast actins due to the absence of histidine 73 methylation.

Probing protein-protein interactions with drag flow: A case study of F-actin and tropomyosin

The paper introduces a drag flow method to study protein-protein interactions, showing that low forces detach tropomyosin clusters from actin filaments, with detachment rates depending on isoform and force magnitude.

Inorganic phosphate in Arp2/3 complex acts as a rapid switch for the stability of actin filament branches

This study shows that inorganic phosphate (Pi) in the Arp2/3 complex stabilizes actin filament branches, with Pi release rates and force-dependent stability affecting branch stability and regrowth.

Continuous self-repair protects vimentin intermediate filaments from fragmentation

This study investigates the mechanism of vimentin filament disassembly, revealing continuous self-repair and subunit exchange dynamics that prevent fragmentation.

Arp2/3-mediated bidirectional actin assembly by SPIN90 dimers in metazoans

The paper reveals that human SPIN90 forms dimers to nucleate bidirectional actin filaments via Arp2/3, with structural insights showing conserved dimerisation in metazoans.

HIV-1 budding requires cortical actin disassembly by the oxidoreductase MICAL1

This study shows that HIV-1 budding requires MICAL1-mediated actin disassembly at the plasma membrane, with Rab35 also playing a role in this process.