Lidiya Lisitskaya

Tetramerization and RNA-guided filament assembly control Schlafen-Argonaute antiphage defense

This study identifies a bacterial antiphage defense system combining Ago and SLFN domains, showing how ATP-driven tetramerization and RNA-guided filament assembly regulate nuclease activity.

Targeting of human mitochondrial DNA with programmable pAgo nuclease

This study demonstrates that a bacterial Argonaute protein can be targeted to mitochondria to reduce mtDNA copy number in human cells.

Target-induced Argonaute-HNH filaments confer bacterial immunity

The paper shows that short prokaryotic Argonautes (pAgos) activate HNH nuclease effectors after RNA-guided DNA recognition, forming filaments that degrade DNA and confer bacterial immunity.

DNA-targeting short Argonaute triggers effector nuclease to protect bacteria from invaders

The paper shows that NbaAgo forms a complex with an effector nuclease to cleave DNA/RNA upon target recognition, providing bacterial immunity against invaders.

Bacterial Argonaute nucleases reveal different modes of DNA targeting in vitro and in vivo

This study investigates the DNA targeting mechanisms of five bacterial Argonaute proteins in vitro and in vivo, revealing their ability to cleave DNA and defend against bacteriophages.