Andrey V. Kulbachinskiy

Tetramerization and RNA-guided filament assembly control Schlafen-Argonaute antiphage defense

This study identifies a bacterial antiphage defense system combining Ago and SLFN domains, showing how ATP-driven tetramerization and RNA-guided filament assembly regulate nuclease activity.

Targeting of human mitochondrial DNA with programmable pAgo nuclease

This study demonstrates that a bacterial Argonaute protein can be targeted to mitochondria to reduce mtDNA copy number in human cells.

DNA-targeting short Argonaute triggers effector nuclease to protect bacteria from invaders

The paper shows that NbaAgo forms a complex with an effector nuclease to cleave DNA/RNA upon target recognition, providing bacterial immunity against invaders.

Bacterial Argonaute nucleases reveal different modes of DNA targeting in vitro and in vivo

This study investigates the DNA targeting mechanisms of five bacterial Argonaute proteins in vitro and in vivo, revealing their ability to cleave DNA and defend against bacteriophages.

Specific targeting of plasmids with Argonaute enables genome editing

This paper demonstrates that CbAgo from Clostridium butyricum can be used for targeted genomic DNA cleavage and recombineering by using plasmid-derived gDNAs.

Bacterial Argonaute proteins aid cell division in the presence of topoisomerase inhibitors in Escherichia coli

The paper shows that bacterial Argonaute proteins assist in cell division in E. coli under topoisomerase inhibitor stress by processing DNA replication termination sites.

A programmable pAgo nuclease with universal guide and target specificity from the mesophilic bacterium Kurthia massiliensis

This paper describes a programmable pAgo nuclease from Kurthia massiliensis that can cleave both DNA and RNA targets with guide flexibility and mismatch sensitivity.

Structural basis of ribosomal RNA transcription regulation

This study reveals how DksA/ppGpp regulates rRNA transcription by altering RNAP conformation and promoter DNA binding in Escherichia coli.

pAgo-induced DNA interference protects bacteria from invader DNA

This study shows that the bacterial Argonaute nuclease CbAgo uses DNA interference to cleave multicopy genetic elements like plasmids and transposons by generating small DNA guides through cooperation with repair machinery.

Programmable DNA cleavage by Ago nucleases from mesophilic bacteria Clostridium butyricum and Limnothrix rosea

This study identifies two mesophilic prokaryotic Argonautes that function as DNA-guided DNA nucleases at physiological temperatures, expanding their potential for genomic applications.

The expanded universe of prokaryotic Argonaute proteins

This study expands the known diversity of prokaryotic Argonaute proteins by analyzing sequenced genomes and identifying new genes, revealing their potential roles in DNA processing and genome editing.