This study identifies SCM-1/SCAMP as a key factor maintaining microdomain boundaries in the endosomal system, ensuring proper cargo sorting and preventing microdomain overlap.

bioRxiv · May 21, 2026 · PreprintCell Biology

Cargo-selective regulation of clathrin-mediated endocytosis by AMP-activated protein kinase

Laura A. Orofiamma, Ralph Christian Delos Santos, Ayshin Mehrabi, Nikol Leshchyshyn, Geoffrey G. Hesketh, Sadia Rahmani, Aesha Dharmesh Patel, Farnaz Fekri, Rehman Ata, Colin D.H. Ratcliffe, Mathieu J. F. Crupi, Lois M. Mulligan, Morag Park, Anne‐Claude Gingras, Costin N. Antonescu

This study shows AMPK regulates clathrin-mediated endocytosis by controlling Dab2 and Arf6 to selectively internalize β1-integrin.

iScience · Jul 17, 2025Peer-reviewedCell Biology

Control of GSK3beta nuclear localization by amino acid signaling requires GATOR1 but is mTORC1 independent

Diana Schwendener Forkel, Osemudiamen Ibazebo, Sumaiya A. Soha, Stephen James Bautista, Stefania Impellizzeri, Roberto J. Botelho, Geoffrey G. Hesketh, Anne‐Claude Gingras, Costin N. Antonescu

The paper shows that GATOR1 regulates GSK3β nuclear localization in response to amino acids, independent of mTORC1, and that this process supports cell survival under metabolic stress.

bioRxiv · Apr 9, 2024 · PreprintCell Biology

A proximity-dependent biotinylation map of a human cell: an interactive web resource

Christopher D. Go, James D.R. Knight, Archita Rajasekharan, Bhavisha Rathod, Geoffrey G. Hesketh, Kento T. Abe, Ji-Young Youn, Payman Samavarchi-Tehrani, Hui Zhang, Lucie Y. Zhu, Evelyn Popiel, Jean-Philippe Lambert, Étienne Coyaud, Sally W.T. Cheung, Dushyandi Rajendran, , Anne‐Claude Gingras

Profiling 192 subcellular markers by proximity-dependent biotinylation (BioID) in human HEK 293 cells maps 4,145 proteins across 32 compartments via 35,902 interactions, available with analysis tools at humancellmap.org.

bioRxiv · Mar 17, 2021 · PreprintMolecular Biology