M. Sloan Siegrist

Metabolic flexibility and an unusual route for peptidoglycan muramic acid recycling in mycobacteria

This study identifies a novel peptidoglycan recycling pathway in mycobacteria that bypasses typical intermediates, revealing metabolic flexibility in cell wall recycling.

Molecular Determinants Governing the Antitubercular Activity of Griselimycin

This study investigates the structure-activity relationship of griselimycin, identifying key amino acids and modifications that enhance its antitubercular activity.

Split-luciferin Assay for Real-time Measurement of Cytosolic Molecular Accumulation in Live Mycobacteria

This paper introduces D-CLAP, a split-luciferin assay to measure real-time cytosolic accumulation of compounds in live mycobacteria, showing that modifying carboxylates increases accumulation and antimycobacterial activity.

Loss of Ag85A disrupts plasma membrane domains and promotes free mycolic acid accumulation in mycobacteria.

This study shows that Ag85A is essential for plasma membrane domain organization in mycobacteria, leading to mycolic acid accumulation and altered cell envelope structure.

Systematic Determination of the Impact of Structural Edits on Peptide Accumulation into Mycobacteria

The paper evaluates how structural modifications like macrocyclization and N-alkylation affect peptide accumulation in Mycobacterium tuberculosis using a novel assay, showing macrocyclization generally enhances accumulation while N-alkylation has context-dependent effects.

The mycomembrane differentially and heterogeneously restricts antibiotic permeation

This paper shows that the mycomembrane of Mycobacterium tuberculosis and M. smegmatis differentially and heterogeneously restricts antibiotic permeation using the PAC-MAN method.

Mycobacterium tuberculosis MutT4 is an RNA pyrophosphohydrolase that forms biomolecular condensates and sensitizes mRNAs to degradation

This study identifies MutT4 in Mycobacterium tuberculosis as the RppH homolog, showing it forms condensates that facilitate mRNA degradation and enhance stress resistance.

Trehalose dimycolate inhibits phagosome maturation and promotes intracellular M. tuberculosis growth via noncanonical SNARE interaction

This study identifies TDM's mechanism of inhibiting phagosome maturation by interacting with SNARE proteins, promoting M. tuberculosis growth.

Identification of chemical features that influence mycomembrane permeation and antitubercular activity

The paper identifies chemical features affecting mycomembrane permeation and antitubercular activity using a bioorthogonal assay and machine learning.

The cell wall polymer initiates plasma membrane partitioning in mycobacteria

The paper shows that the cell wall polymer, not active polymerization, is critical for membrane partitioning in Mycobacterium smegmatis.