Fiona Brown

NEK1 autophosphorylation is disrupted by amyotrophic lateral sclerosis-associated missense variants: activity biomarkers and structural insights

Kinase-dead controls, XIC quantification and phosphospecific antibodies establish pSer14, pThr156 and pSer418 as NEK1 autophosphorylation sites; a pThr156 assay shows ALS missense variants differ, with R261C reducing it most.

Phosphorylation enables progressive microtubule-associated protein proteolysis and functionalisation during neural development

The paper identifies SRPK-mediated phosphorylation of MAP1S, showing it regulates proteolytic processing and microtubule binding during neurodevelopment.

NRBP1 pseudokinase binds to and activates the WNK pathway in response to osmotic stress

This study identifies NRBP1 as an upstream activator of the WNK pathway by forming a multi-subunit complex with WNK1, SPAK, and TSC22D4 in response to osmotic stress.