Antonio Rios

Scalable multimodal mapping of macrophage regulatory architecture by integrating optical and transcriptomic pooled screens

The paper introduces PerturbPair, a platform integrating transcriptomic and optical screens to map macrophage regulatory networks, revealing cross-modal relationships and disease-relevant gene programs.

Degron-modified Cas12a enhances single-cell CRISPR screening

This paper develops a degron-modified Cas12a system to enable accurate single-cell detection of pre-crRNAs, enhancing Perturb-seq capabilities for multiplex guide expression.

Highly multiplexed, image-based pooled screens in primary cells and tissues with PerturbView

PerturbView enables highly multiplexed optical pooled screens in primary cells and tissues by improving barcode amplification, revealing regulators of NFκB signaling and enabling spatial transcriptomics integration.

Sequential Optimal Experimental Design of Perturbation Screens Guided by Multi-modal Priors

This paper introduces an active learning method to efficiently design Perturb-seq experiments by leveraging multi-modal priors, improving perturbation space exploration with fewer experiments.

Enhanced Cas12a multi-gene regulation using a CRISPR array separator

The paper identifies a CRISPR separator that improves Cas12a array performance by reducing crRNA interference, enabling enhanced multi-gene regulation in human cells.