Valentina Snetkova

Advancing the genetic engineering toolbox by combining AsCas12a knock-in mice with ultra-compact screening

The paper introduces an enAsCas12a mouse model and associated libraries for efficient gene editing and screening, identifying drug resistance genes and enabling simultaneous gene knockouts and activations.

Degron-modified Cas12a enhances single-cell CRISPR screening

This paper develops a degron-modified Cas12a system to enable accurate single-cell detection of pre-crRNAs, enhancing Perturb-seq capabilities for multiplex guide expression.

Increased Enhancer-Promoter Interactions during Developmental Enhancer Activation in Mammals

The paper examines 3D enhancer-promoter interactions during mammalian development, finding that most enhancers bypass neighboring genes and show tissue-specific conformations with increased interactions upon activation.

EpiMethylTag simultaneously detects ATAC-seq or ChIP-seq signals with DNA methylation

EpiMethylTag combines ATAC-seq or ChIP-seq with bisulfite conversion to simultaneously assess chromatin accessibility/TF binding and DNA methylation.

Context-dependent requirement of H3K9 methyltransferase activity during cellular reprogramming to iPSCs

This study shows that reducing EHMT1/2 activity during reprogramming with ascorbic acid impairs iPSC formation and viability, highlighting the role of H3K9 methylation in reprogramming efficiency.

The interdependent nature of multi-loci associations can be revealed by 4C-Seq

The paper introduces a method to identify multi-loci chromatin interactions using 4C-seq data and demonstrates its application to Tcrb and Igk enhancer interactions.

4C-ker: A method to reproducibly identify genome-wide interactions captured by 4C-Seq experiments

This paper introduces 4C-ker, a method that improves the reproducibility of identifying genome-wide interactions from 4C-Seq data by accounting for signal biases and varying resolution.