Lei S. Qi

The paper introduces DARTs, a platform for programmable cell targeting using antibody-conjugated nanoparticles, identifying new surface antigens for gene editing in neural cells.

PHAROS uses a pretrained single-cell perturbation model to predict and prioritize effective drug combinations without retraining, showing success in cell lines and patient-derived tumors.

This paper creates a super-resolution atlas of SARS-CoV-2 infection, revealing protease-dependent organelle maturation, dsRNA structures, and structural proteins, with implications for antiviral strategies.

This study demonstrates a programmable macromolecule delivery system using engineered trogocytosis to transfer proteins between cells, enabling genome editing and targeted cell ablation.

The paper identifies RASGRP3 as a context-specific essential gene in uveal melanoma and develops a CRISPR-Cas13d RNA-targeting therapeutic that selectively eliminates uveal melanoma cells.

This paper develops a viral riboswitch system for real-time coronavirus detection and antiviral response in live cells, showing effectiveness against 229E and OC43.

This study introduces CRISPR-TO to manipulate RNA localization in neurons, showing it affects neurite outgrowth and axonal regeneration.

The paper introduces a method to control cell fates by modulating multiple genes using CRISPR tools and machine learning, demonstrating its effectiveness in stem cell differentiation.

The paper develops HT-recruit to measure transcriptional effector function across contexts and identifies context-dependent effectors for CRISPR-based transcription control.

This paper shows that CRISPRa activation of two endogenous regulatory elements can self-organize ESCs into embryo-like structures with spatial and molecular features similar to pre-gastrulation mouse embryos.

This paper demonstrates that sadCas9-mediated CRISPRi reduces alpha-synuclein expression in a humanized mouse model of Parkinson's disease, showing long-term downregulation and attenuated immune response.

The paper identifies compact CRISPR-based activators with durable gene activation, improving upon existing tools in potency and persistence.

This study uses CRISPRi to downregulate alpha-synuclein in a Parkinson's disease patient-derived model, reducing oxidative stress and mtDNA damage.

This paper develops valency-controlled receptors (VCRs) to enhance CAR functionality by modulating T cell signaling through multivalent ligands, showing improved immune response against tumors.

The paper identifies a CRISPR separator that improves Cas12a array performance by reducing crRNA interference, enabling enhanced multi-gene regulation in human cells.

This study uses CRISPR-EChO to investigate HP1's role in organizing chromatin at supranucleosomal scales, revealing its ability to compact chromatin and repress transcription over large genomic regions.

The paper describes a reporter system to quantify durable CRISPR-based gene silencing and reports a single CRISPR protein capable of producing long-lasting gene repression.

This study develops a CRISPR/Cas9 method to reversibly disrupt specific transcription factor-DNA interactions and assess their functional impact on gene expression and cell division.

This paper introduces CRISPR ChaCha, a GPCR-Coupled CRISPR-Cas system that converts extracellular signals into genetic programs, showing improved performance over existing systems.

The paper introduces a method to multiplex label and track genomic loci in single cells using CRISPR imaging combined with DNA seqFISH, demonstrating it on telomere dynamics in mouse embryonic stem cells.